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Anti-Na+/H+ Exchanger 7 (NHE7) (extracellular) Antibody

Sodium/hydrogen exchanger 7, Solute Carrier Family 9 Member A7, SLC9A7, NHE-7

Cat #: ANX-007
Alternative Name Sodium/hydrogen exchanger 7, Solute Carrier Family 9 Member A7, SLC9A7, NHE-7
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EISPGKINNVEQND, corresponding to amino acid residues 158 - 171 of mouse SLC9A7 (Accession Q96T83). Extracellular, 2nd loop.
Accession (Uniprot) Number Q96T83
Gene ID 84679
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – identical; Human - 13 out of 14 amino acid residues identical
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1-2. Anti-Na+/H+ Exchanger 7 (NHE7) (extracellular) Antibody (#ANX-007), (1:200).
    3-4. Anti-Na+/H+ Exchanger 7 (NHE7) (extracellular) Antibody, preincubated with Na+/H+ Exchanger 7 (NHE7) (extracellular) Blocking Peptide (BLP-NX007).
  • Western blot analysis of human U-87 MG glioma cell line lysate:
    Western blot analysis of human U-87 MG glioma cell line lysate:
    1. Anti-Na+/H+ Exchanger 7 (NHE7) (extracellular) Antibody (#ANX-007), (1:200).
    2. Anti-Na+/H+ Exchanger 7 (NHE7) (extracellular) Antibody, preincubated with Na+/H+ Exchanger 7 (NHE7) (extracellular) Blocking Peptide (BLP-NX007).
Immunohistochemistry
  • Expression of NHE7 in mouse hippocampus.
    Expression of NHE7 in mouse hippocampus.
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Na+/H+ Exchanger 7 (NHE7) (extracellular) Antibody (#ANX-007), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal CA3 region, showed immunoreactivity of NHE7 (green) in the CA3 pyramidal layer (arrows). B. Pre-incubation of the antibody with Na+/H+ Exchanger 7 (NHE7) (extracellular) Blocking Peptide (BLP-NX007), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Fuster, D.G., et al (2014) Pflugers Arch - Eur J Physiol 466, 61.
  2. Lin PJ. J et al (2005) Cell Sci. 118, 1885.
  3. Khayat, W., et al (2019) Hum Mol Genet 28(4), 598.
Scientific background

Na+/H+ exchangers (NHEs) also termed solute carrier family 9 (SLC9), are proteins that function as ion transporters. NHEs are present across all kingdoms of life from prokaryotes to eukaryotes and are found to be crucial in preventing intracellular acidification. NHEs are divided into 3 groups SLC9A (NHE1-9), SLC9B (NHA1 and NHA2) and SLC9C according to their cellular location and their function. NHEs regulates cell volume and pH of the cytosol and organelles1.

NHE7 is an NHE that is localized predominantly to the trans-Golgi network (TGN) and post-Golgi vesicles. NHE7 differ from other NHEs by its ability to transport both Na+ and K+ via a H+ gradient-dependent manner2. NHE7 mRNA is expressed in wide range of cells but is especially abundant in the brain, skeletal muscle and various secretory tissues3.

The predicted membrane topology of NHE7 consist of 12 transmembrane domains (TMDs) and a single N-glycosylation site at position 145. Similarly to the other SLC9A isoforms NHE7 can be assembled as a homodimer. It was recently found that a missense variant in NHE7 associates with multigenerational nonsyndromic intellectual disability (ID)3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 31/08/2021

Anti-Na+/H+ Exchanger 7 (NHE7) (extracellular) Antibody (#ANX-007) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NHE7 from rat, mouse and human samples.

For research purposes only, not for human use
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