Anti-NCS1-ATTO-594 Antibody

Neuronal calcium sensor 1, Frequenin homolog, Frequenin-like protein, FREQ, FLUP
  • New
Cat #: ACS-001-AR
Alternative Name Neuronal calcium sensor 1, Frequenin homolog, Frequenin-like protein, FREQ, FLUP
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)SGQLDAAGFQKIYK, corresponding to amino acid residues 40-53 of rat NCS1 (Accession P62168). Intracellular.
    Accession (Uniprot) Number P62168
    Gene ID 65153
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 – 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACS-001), and immunohistochemistry (labeled antibody).
    Applications: ih
    May also work in: ic
      • Expression of Neuronal calcium sensor 1 in mouse brain
        Immunohistochemical staining of mouse brain sections using Anti-NCS1-ATTO-594 Antibody (#ACS-001-AR). A. In the cerebellum, NCS1 immunostaining (red) appears in the Purkinje cell layer (P), (arrows). B. In the hippocampus NCS1 (red) appears in CA3 pyramidal layer (arrows). Nuclei are stained using DAPI (blue).
      • Neuronal calcium sensor-1 (NCS-1) is a member of the neuronal calcium sensor family of EF-hand Ca2+-binding proteins that triggers different biological processes to regulate signal transduction in neurons and photoreceptor cells. This family of proteins includes 15 members and is primarily expressed in neurons. 

        The protein interacts with the G-protein coupled receptor (GPCR) Dopamine D2 receptor (D2R), and the cognate kinases GRK1 and GRK2. NCS-1 responds to changes in intracellular Ca2+ concentration and is involved in many important physiological functions including the regulation of neurotransmitter release, synaptic plasticity, learning and memory, neurite growth, and neuronal survival1,2.

        NCS-1 structure is characterized by 4 helix-loop-helix motifs, called EF-hand motifs able to bind Ca2+ ions. These domains are separated by a short loop containing a conserved glycine residue. NCS-1 is targeted to the plasma membrane by an N-terminal myristoylation group through the action of N-miristoyltransferase. Opposite to the EF-motifs is a wide hydrophobic crevice (HC). This area has the ability to interact with various physiological targets2,3.

        NCS-1 dysfunctions are linked to mental conditions, such as bipolar disorder, schizophrenia and autism2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 12/04/2019

    Anti-NCS1 Antibody (#ACS-001) is a highly specific antibody directed against an epitope of the rat Neuronal calcium sensor 1. The antibody can be used in western blot analysis. It has been designed to recognize NCS1 from rat, mouse, and human samples.

    Anti-NCS1-ATTO-594 Antibody (#ACS-001-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-NCS1-ATTO-594 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use