Anti-Neurokinin Receptor 1 (NK1) (extracellular)-ATTO-488 Antibody

NK1 Tachykinin receptor, Substance-P receptor, Neurokinin1 receptor, Tachykinin receptor 1
    Cat #: ATR-001-AG
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide CMIEWPEHPNRTYEK, corresponding to amino acid residues 180-194 of rat Neurokinin Receptor 1 (NK1) (Accession P14600). 2nd extracellular loop.
    Accession (Uniprot) Number P14600
    Gene ID 24807
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 13/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 mg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ATR-001), and immunocytochemistry (labeled antibody).
    Applications: ih
    May also work in: ic, lci
    Immunohistochemistry
    Expression of NK1 in rat colon
    Immunohistochemical staining of rat colon paraffin-embedded section using Anti-Neurokinin Receptor 1 (NK1) (extracellular)-ATTO-488 Antibody (#ATR-001-AG), (1:100). A. NK1 labeling (green) appears in the tubular glands of the mucosa layer. B. Nuclear staining using DAPI. C. Merge of panels A. and B.
    Immuno-colocalization of VGLUT2 and Neurokinin Receptor 1 in rat DRG
    Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglion (DRG) sections using Anti-VGLUT2-ATTO-594 Antibody (#AGC-036-AR), (1:60) and Anti-Neurokinin Receptor 1 (NK1) (extracellular)-ATTO-488 Antibody (#ATR-001-AG), (1:60). A. VGLUT2 staining (red). B. Neurokinin Receptor 1 staining (green). C. Merge of the two images demonstrates co-localization in some neuronal bodies (arrows point at examples). Cell nuclei are stained with DAPI (blue).
    Immuno-colocalization of NK1 and mGluR5 in rat DRG
    Immunohistochemical staining of rat dorsal root ganglion using Anti-Neurokinin Receptor 1 (NK1) (extracellular)-ATTO-488 Antibody (#ATR-001-AG), (green), (1:60) and Anti-mGluR5 (extracellular)-ATTO-594 Antibody (#AGC-007-AR), (red), (1:60).  A. NK1staining. B. mGluR5 staining appears in both cells (horizontal arrow) and fibers (vertical arrow).   C. Merge of A and B demonstrates the co-localization of NK1 and mGluR5 receptors. Nuclei staining using DAPI as the counterstain (blue).
    References
    1. Pennefather, J.N. et al. (2004) Life Sci. 74, 1445.
    2. Hershey, A.D. et al. (1991) J. Biol. Chem. 266, 4366.
    3. Gerard, N.P. et al. (1993) Regul. Pept. 43, 21.
    4. Caberlotto, L. et al. (2003) Eur. J. Neurosci. 17, 1736.
    5. Munoz, M. et al. (2011) Expert Opin. Ther. Targets 15, 889.
    6. Ebner, K. et al. (2009) Curr. Pharm. Des. 15, 1647.
    7. May, A. and Goadsby, P.J. (2001) Expert Opin. Investig. Drugs 10, 673.
    8. Palecek, J. et al. (2003) Neuroscience 116, 565.
    Scientific background

    Substance P (SP), Neurokinin A (NKA) and Neurokinin B (NKB) are all peptides belonging to the Tachykinin protein family. These three peptides which demonstrate a quite heterogeneity in their distribution exert their effect via three receptors: Neurokinin 1-3 receptors, members of the G-protein coupled receptor superfamily. However, Neurokinin 1 Receptor (NK1) preferentially binds Substance P, Neurokinin 2 Receptor (NK2) to NKA and Neurokinin 3 Receptor (NK3) to NKB1.

    Neurokinin receptors are distinguished by their seven transmembrane domains, an extracellular N-terminus and a cytosolic C-terminal. An unusual property of these receptors is the presence of introns as part of their structural organization1-3. Tachykinin receptors undergo alternative splicing. For example, NK1 is detected with different C-terminal lengths. The longer receptor isoform is found in the brain whereas the truncated form is mostly detected in the periphery1,4.

    Due to the broad expression profile of tachykinin peptides, their respective receptors are also expressed in a similar fashion. NK1 is widely expressed in neurons endothelial cells, muscle and immune system cells. NK2 is broadly expressed in the periphery and its expression in the brain is quite restricted. NK3 on the other hand is largely expressed in the central nervous system and is also detected in the uterus, skeletal muscle, lung and liver1.

    Neurokinin receptors have been found in many pathophysiological indications and have therefore become targets for the development of pharmacological compounds. Such indications include cancer, psychological disorders, migraine and various inflammations, just to name a few5-8.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Immuno-colocalization of Noradrenaline Transporter (NET) and Neurokinin Receptor 1 (NK1) in rat brain stem.Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Noradrenaline Transporter (NET) (extracellular) Antibody (#AMT-002), (1:400) and Anti-Neurokinin Receptor 1 (NK1) (extracellular)-ATTO-488 Antibody (#ATR-001-AG), (1:80). A. NET staining (red) in section of rat locus coeruleus. B. NK1 staining (green) in same section. C. Merge of the two images reveals several cells expressing both NET and NK1 (arrows). Cell nuclei are stained with DAPI (blue).

    Last update: 24/10/2018

    Anti-Neurokinin Receptor 1 (NK1) (extracellular) Antibody (#ATR-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect live cell flow cytometry, and live cell imaging applications. It has been designed to recognize NK1 from rat, mouse, and human samples.

    Anti-Neurokinin Receptor 1 (NK1) (extracellular)-ATTO-488 Antibody (#ATR-001-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Neurokinin Receptor 1 (NK1) (extracellular)-ATTO-488 Antibody is especially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use
    Citations
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