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Anti-NGL-3/LRRC4B (extracellular) Antibody

Netrin-G ligand 3, Netrin-G3 ligand, Leucine-rich repeat-containing protein 4B, LRIG4

Cat #: ANR-163
Alternative Name Netrin-G ligand 3, Netrin-G3 ligand, Leucine-rich repeat-containing protein 4B, LRIG4
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)PGEEAQQPRGTEKE, corresponding to amino acid residues 498-511 of mouse NGL-3 (Accession P0C192). Extracellular, N-terminus.
Accession (Uniprot) Number P0C192
Gene ID 272381
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – identical; human – 13 out of 14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat new born brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
    Western blot analysis of rat new born brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
    1,2. Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163), (1:200).
    3,4. Anti-NGL-3/LRRC4B (extracellular) Antibody, preincubated with NGL-3/LRRC4B (extracellular) Blocking Peptide (#BLP-NR163).
  • Western blot analysis of mouse brain lysate:
    Western blot analysis of mouse brain lysate:
    1. Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163), (1:200).
    2. Anti-NGL-3/LRRC4B (extracellular) Antibody, preincubated with NGL-3/LRRC4B (extracellular) Blocking Peptide (#BLP-NR163).
Immunohistochemistry
  • Expression of NGL-3 in mouse hippocampus
    Expression of NGL-3 in mouse hippocampus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163), (1:200), followed by goat anti-rabbit-AlexaFluor-488. NGL-3 immunoreactivity (green) is detected in the pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).
References
  1. Seiradake, E. et al. (2011) EMBO J. 30, 4479.
  2. Kwon, S.K. et al. (2010) Biol. Chem. 285, 13966.
  3. Lin, J.C. et al. (2003) Neurosci. 6, 1270.
  4. Kim, S. et al. (2006) Neurosci. 9, 1294.
  5. Woo, J. et al. (2009) Neurosci. 12, 428.
  6. Lee, H. et al. (2019) PLoS Biol. 17, e2005326.
Scientific background

NGL-3 (Netrin G ligand 3) is a postsynaptic cell adhesion molecule that is a member of the NGL family. NGLs are proteins involved in the regulation of various steps in synapse formation. Members of this family share a common domain structure that contains: nine Leucine rich repeats (LRRs), an immunoglobulin (Ig) domain in the extracellular region, a single trans membrane domain and a cytoplasmic region that ends with a PDZ domain-binding motif. This motif mediates the interaction with PSD-95, an abundant postsynaptic scaffolding protein1,2.

The NGL family is comprised of three proteins: NGL-1, 2, and 3. NGL-1 and NGL-2 interact through their extracellular domain with the GPI anchored membrane proteins Netrin G1 and G2 respectively2-4. This interaction is important for regulation of structural and functional excitatory synapse development4.

NGL-3 trans-synaptically interacts with the leukocyte common antigen-related (LAR) protein which is well-known for its involvement in axon guidance and presynaptic differentiation. This interaction induces pre- and postsynaptic differentiation in contacting axons and dendrites, respectively, and regulates excitatory synapse formation2,5.

Mice lacking NGL-3 (Ngl3−/−) show markedly suppressed normal brain development and postnatal survival and growth6. NGL-3 overexpression and knockdown in cultured neurons result in bidirectional changes in the number of excitatory synapses and spontaneous excitatory synaptic transmission5.

NGL-3 mRNA and proteins are mainly detected in synaptic fractions of the brain and levels gradually increase during the first 3 weeks of postnatal rat brain development5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect the protein in living cells. It has been designed to recognize LRRC4B from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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