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Anti-Slitrk1 (extracellular) Antibody

SLIT and NTRK-like family member 1, Leucine-rich repeat-containing protein 12, LRRC12
Cat #: ASR-081
Alternative Name SLIT and NTRK-like family member 1, Leucine-rich repeat-containing protein 12, LRRC12
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RDNKIHSIRKSHFVD, corresponding to amino acid residues 383-397 of mouse Slitrk1 (Accession Q810C1). Extracellular, N-terminus.
Accession (Uniprot) Number Q810C1
Gene ID 76965
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human – identical; rat – 14/15 amino acid residues identical.
RRID AB_2756800.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain synaptosomal fraction (lanes 2 and 4):
    Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain synaptosomal fraction (lanes 2 and 4):
    1,2. Anti-Slitrk1 (extracellular) Antibody (#ASR-081), (1:200).
    3,4. Anti-Slitrk1 (extracellular) Antibody, preincubated with Slitrk1 (extracellular) Blocking Peptide (#BLP-SR081).
Immunohistochemistry
  • Multiplex staining of Slitrk1 and KV2.1 in rat parietal cortex
    Multiplex staining of Slitrk1 and KV2.1 in rat parietal cortex
    Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using rabbit Anti-Slitrk1 (extracellular) Antibody (#ASR-081), (1:200) and Guinea pig Anti-KV2.1 Antibody (#AGP-109), (1:200). A. Slitrk1 staining (red) appears in profiles of pyramidal neurons. B. KV2.1 staining (green) is detected in profiles of pyramidal neurons. C. Merge of the two images shows colocalization in several neurons (arrows). Cell nuclei are stained with DAPI (blue).
  • Expression of Slitrk1 in rat cortex
    Expression of Slitrk1 in rat cortex
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Slitrk1 (extracellular) Antibody (#ASR-081), (1:300), followed by goat anti-rabbit-AlexaFluor-488. Slitrk1 staining (green) in the rat parieto-temporal cortex is detected in pyramidal neurons (horizontal arrow) and their dendrites (vertical arrow). Cell nuclei are stained with DAPI (blue).
References
  1. Aruga, J. and Mikoshiba, K. (2003) Mol. Cell. Neurosci. 24, 117.
  2. Beaubien, F. et al. (2016) Sci. Rep. 6, 27343.
Scientific background

Neurites are key morphological features of neurons, and are classified into axons and dendrites. Leucine-rich repeat (LRR) domains are often found in neurite development-controlling proteins. Slitrk proteins are leucine-rich repeat containing transmembrane proteins, parts of which are similar to Slit and neurotrophin receptor.

All six members of the Slitrk family, including Slitrk1, contain putative hydrophobic signal sequences and membrane-spanning regions. Slitrk1 is strongly expressed in the subventricular zone of the cerebral cortex, the pyramidal cell layer of the hippocampus and in the thalamus and hypothalamus1.

Slitrk1, can regulate synapse formation between hippocampal neurons. Slitrk1 is enriched in postsynaptic fractions and is localized to excitatory synapses. Overexpression of Slitrk1 in hippocampal neurons increases the number of synaptic contacts on these neurons. Furthermore, decreased expression of Slitrk1 in hippocampal neurons leads to a reduction in the number of excitatory, but not inhibitory, synapses formed in hippocampal neuron cultures. In addition, different leucine rich repeat domains of the extracellular region of Slitrk1 are necessary to mediate interactions with Slitrk binding partners of the LAR receptor protein tyrosine phosphatase family, and to promote dimerization of Slitrk1. In addition, Slitrk1 binds with PSD-95 through its intracellular tail. Thus, through PSD-95, Slitrk1 regulates signaling of NMDA and AMPA receptors2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-Slitrk1 (extracellular) Antibody (#ASR-081) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize Slitrk1 from mouse, human, and rat samples.

For research purposes only, not for human use
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