Anti-Pannexin 2 (extracellular) Antibody

PANX2
    Cat #: ACC-232
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)EEPIY(S)YTPHNFTRD, corresponding to amino acid residues 76-90 of human Pannexin 2 (Accession Q96RD6). 1st extracellular loop.
    Accession (Uniprot) Number Q96RD6
    Gene ID 56666
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, mouse, rat - 14/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, lci, wb
    May also work in: ifc, ih, ip
    Western blot
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1, 2.  Anti-Pannexin 2 (extracellular) Antibody (#ACC-232), (1:200).
    3, 4.  Anti-Pannexin 2 (extracellular) Antibody, preincubated with the control peptide antigen.
    Live cell imaging / Immunocytochemistry
    Expression of Pannexin 2 in human U-87 MG glioblastoma cell line
    Immunocytochemical staining of live intact human U-87 MG glioblastoma cells. A. Cells were stained with Anti-Pannexin 2 (extracellular) Antibody (#ACC-232), (1:50), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.
    References
    1. Chew, S.S. et al. (2010) Exp. Neurol. 225, 250.
    2. Nakagawa, S. et al. (2010) Curr. Opin. Struct. Biol. 20, 423.
    3. Trexler, E.B. et al. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 5836.
    4. Hu, X. et al. (2006) Biophys. J. 90, 140.
    5. Shestopalov, V.I. and Panchin, Y. (2008) Cell. Mol. Life Sci. 65, 376.
    6. Unger, V.M. et al. (1999) Science 283, 1176.
    7. Falk, MM. (2000) Eur. J. Cell. Biol. 79, 564.
    8. Panchin, Y.V. (2005) J. Exp. Biol. 208, 1415.
    9. Litvin, O. et al. (2006) J. Cell. Mol. Med. 10, 613.
    10. Bruzzone, R. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 13644.
    Scientific background

    Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells1. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2-4.

    Proteins involved in gap junction formation are composed of four transmembrane domains, 2 extracellular loops and one intracellular loop and intracellular N- and C-termini5,6. Several consensus cysteine residues are in the extracellular loop and are essential and necessary for intercellular docking of gap junction hemichannels in the opposing cell membrane5-7.

    Pannexins (Pannexin 1, Pannexin 2 and Pannexin 3) belong to the superfamily of gap junction proteins. Pannexin 1 (PANX1) is ubiquitously expressed, Pannexin 2 (PANX2) is specifically expressed in the human brain and widespread in rodents and Pannexin 3 (PANX3) is also detected in the brain8.

    The gating properties of Pannexins were studied in Xenopus oocytes and results demonstrate that only PANX1 is able to form homomeric hemichannels, and is also able to form heteromeric hemichannels with PANX2 but not with PANX39,10. Not surprising, PANX1 gating properties depend whether it forms homomeric or heteromeric hemichannels.

    A number of different stimuli are known to open these channels and include mechanical stress, extracellular ATP, increases in intracellular Ca2+ and inflammation9.

    Possible roles for Pannexins include paracrine signaling in vascular endothelial cells and taste cell signaling9.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 02/12/2018

    Anti-Pannexin 2 (extracellular) Antibody (#ACC-232) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize PANX2 from rat, mouse, and human samples.

    For research purposes only, not for human use