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Anti-Piezo2 Antibody

Piezo-Type Mechanosensitive Ion Channel Component 2, Protein FAM38B
Cat #: APC-090
Alternative Name Piezo-Type Mechanosensitive Ion Channel Component 2, Protein FAM38B
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RTIFHDITRLHLD, corresponding to amino acid residues 1092 - 1104 of human Piezo2 (Accession Q9H5I5). The peptide is located in an internal region of human Piezo2 (the exact topology of Piezo2 remains to be defined).
Accession (Uniprot) Number Q9H5I5
Gene ID 63895
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat – 12/13 amino acid residues identical.
RRID AB_2876842
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 3) and mouse lung lysate (lanes 2 and 4):
    Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 3) and mouse lung lysate (lanes 2 and 4):
    1, 2. Anti-Piezo2 Antibody (#APC-090), (1:200).
    3, 4. Anti-Piezo2 Antibody, preincubated with Piezo2 Blocking Peptide (#BLP-PC090).
  • Western blot analysis of human HepG2 hepatocellular cell line lysate (lanes 1 and 3) and human HUVEC endothelial cell line lysate (lanes 2 and 4):
    Western blot analysis of human HepG2 hepatocellular cell line lysate (lanes 1 and 3) and human HUVEC endothelial cell line lysate (lanes 2 and 4):
    1, 2. Anti-Piezo2 Antibody (#APC-090), (1:200).
    3, 4. Anti-Piezo2 Antibody, preincubated with Piezo2 Blocking Peptide (#BLP-PC090).
References
  1. Wu, J. et al. (2017) Trends Biochem. Sci. 42, 57.
  2. Wang, L. et al. (2019) Nature 573, 225.
  3. Coste, B. et al. (2010) Science 330, 55.
  4. Ridone, P. et al. (2019) Biophys. Rev. 11, 795.
Scientific background

Piezo-type mechanosensitive ion channel component 2 (Piezo2) is a homotrimeric ion channel. Piezo2 depicts rapidly adapting mechanically activated (MA) currents in somatosensory neurons.

Piezo1 and Piezo2 comprise the Piezo protein family. These are large transmembrane proteins are conserved among various species, and all have between 24 and 36 predicted transmembrane domains. Both channels open in response to mechanical stimuli, allowing positively charged ions, including calcium, to flow into the cell1.

Piezo2 protein contains over 2,800 residues; it forms a three-bladed, propeller like trimer that comprises 114 transmembrane helices (38 per protomer). Transmembrane helices 1–36 (TM1–36) are folded into nine tandem units of four transmembrane helices each to form the unusual non-planar blades2.

Piezo2 has an essential role in sensing gentle touch, tactile pain, airway stretch and lung inflation. It also serves as a baroreceptor for sensing blood pressure and regulating heart rate. Mutations in human Piezo2 are associated with distal arthrogryposis, a disorder characterized by joint deformities (contractures) that restrict movement in the hands and feet3,4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 20/10/2020

Anti-Piezo2 Antibody (#APC-090) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize Piezo2 from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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