Anti-Piezo1 Antibody

Piezo-type mechanosensitive ion channel component 1, Protein FAM38A, Mib, Membrane protein induced by beta-amyloid treatment
Cat #: APC-087
Alternative Name Piezo-type mechanosensitive ion channel component 1, Protein FAM38A, Mib, Membrane protein induced by beta-amyloid treatment
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
      • Peptide (C)EDLKPQHRRHISIR, corresponding to amino acid residues 1863-1876 of rat Piezo1 (Accession Q0KL00). The peptide is located in an internal region of the rat Piezo1 (the exact topology of Piezo1 remains to be defined).
    Accession (Uniprot) Number Q0KL00
    Gene ID 361430
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 12/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity Not recommended for use with human samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
      • Western blot analysis of rat lung (lanes 1 and 3) and mouse lung (lanes 2 and 4) lysates:
        1,2. Anti-Piezo1 Antibody (#APC-087), (1:600).
        3,4. Anti-Piezo1 Antibody, preincubated with the control peptide antigen.
      • Expression of Piezo1 in rat olfactory bulb
        Immunohistochemical staining of rat olfactory bulb sections using Anti-Piezo1 Antibody (#APC-087). A. Piezo1 staining (green) appears in the neuropil with varying intensity within and between glomeruli. B. Nuclear staining (blue) using DAPI as the counterstain. C. Merged image of panels A and B.
      • Piezo1 protein is a family member of mixed cationic, non selective, directly mechanically activated ion channels found in higher eukaryotic cells. Piezo proteins are associated with physiological responses in cells that have been identified in many cell types and tissues in several species where mechanosensitivity plays a major role1,2.

        Two mammalian Piezo proteins were identified, piezo1 and piezo2 encoded by the fam38A and fam38B genes recpectively2. Piezo1 expression is detected in organs such as: bladder, colon, kidney, lung and skin3.

        The secondary structure and general length of Piezo proteins are quite conserved among species, and seem to form homotetrameric aggregates. Each subunit comprises between 24-32 putative transmembrane domains.  The channels are constitutively active in liposomes and their activity does not require any other proteins1.

        Mechanosensitive proteins have been linked to various pathophysiological situations such as hearing and deafness, cardiac physiological and hypertrophic responses, and kidney function and disease2.

        Several studies have demonstrated that Piezo1 is essential for the removal of extra cells without apoptosis. Mutations in Piezo1 protein have been linked to the pathological response of red blood cells in a genetic disease called Xerocytosis. These finding suggest that Piezo1 plays a major role in cells’ responses to mechanical stimuli1. In addition, Piezo1 plays a critical role in endothelial cell mechanotransduction which is essential for embryonic development. Knock-out model of Piezo1 affects the ability of endothelial cells to alter their alignment when exposed to shear stress. Furthermore, loss of Piezo1 in endothelial cells leads to deficits in stress fiber and cellular orientation in response to shear stress, therefore Piezo1mechanotransduction is linked to regulation of cell morphology4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-Piezo1 Antibody
    Knockout validation of Anti-Piezo1 Antibody in mouse pancreas.Immunohistochemical staining of mouse pancreas sections using Anti-Piezo1 Antibody (#APC-087). Piezo1 staining (red) is detected on acinar cells. Piezo1 is not detected in Piezo1-/- conditional knockout mice (right panel).Adapted from Romac, J.M.J. et al. (2018) Nat. Commun. 9, 1715. with permission of SPRINGER NATURE.

    Last update: 20/05/2019

    Anti-Piezo1 Antibody (#APC-087) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Piezo1 from rat and mouse samples. The antibody is unlikely to recognize the protein from human samples.

    For research purposes only, not for human use
    Citations
      • Immunohistochemical staining of mouse pancreas sections. Also tested in Piezo1-/- conditional knockout mice.
        Romac, J.M.J. et al. (2018) Nat. Commun. 9, 1715.
      • Rat bladder lysate.
        Liu, Q. et al. (2018) Exp. Mol. Med. 50, 60.
      • Rat DRG lysate.
        Copp, S.W. et al. (2016) Am. J. Physiol. 310, H1223.
      • Rat bladder sections.
        Liu, Q. et al. (2018) Exp. Mol. Med. 50, 60.
      • Mouse pancreas sections. Also tested in Piezo1-/- conditional knockout mice.
        Romac, J.M.J. et al. (2018) Nat. Commun. 9, 1715.
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