- Peptide (C)KDKSKSGMISGLD, corresponding to amino acid residues 169-181 of mouse SLC25A12 (Accession Q8BH59). Intracellular, N-terminus.
- Western blot analysis of rat skeletal muscle lysate (lanes 1 and 4, 1:600), mouse brain membrane (lanes 2 and 5) and human OVCAR3 ovarian adenocarcinoma cell line lysate (lanes 3 and 6):1-3. Anti-SLC25A12 Antibody (#ANT-112), (1:200).
4-6. Anti-SLC25A12 Antibody, preincubated with SLC25A12 Blocking Peptide (#BLP-NT112).
- Expression of SLC25A12 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC25A12 Antibody (#ANT-112), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal dentate gyrus region, showed immunoreactivity (green) in neurons of the hilus (H, arrows). B. Pre-incubation of the antibody with SLC25A12 Blocking Peptide (#BLP-NT112), suppressed staining. Cell nuclei are stained with DAPI (blue).
G = granule layer.
SLC25 is a large family of nuclear-encoded transporters embedded in the inner mitochondrial membrane and in a few cases other organelle membranes.
SLC2512A is a gene encoding the aspartate/glutamate carrier 1 (AGC1). AGC1 possesses six transmembrane alpha helices and N- and C-termini on the cytosolic side of the membrane1.
AGC1 catalyzes an exchange between intra-mitochondrial aspartate and cytosolic glutamate, an important step in urea synthesis. As a component of the malate–aspartate shuttle (MAS), AGC1 also has a role in the transfer of NADH, carrying reducing equivalents from the cytosol into the mitochondrial matrix. AGC1 is expressed predominantly in heart and skeletal muscle, with weaker expression in brain and kidney. AGC1 is the main AGC isoform present in the adult brain, and it is expressed mainly in neurons. As part of the MAS, AGC1 has a role in the physiology of pancreatic β-cells by influencing glucose-induced activation of mitochondrial metabolism and insulin secretion and it is the only AGC isoform expressed in pancreatic islets and β-cells.
AGC1 has several Ca2+-binding EF-hand motifs located on its long, hydrophilic amino-terminus which extends into the intermembrane space and its activity is strongly regulated by extra-mitochondrial Ca2+. Dysregulation of cellular Ca2+ homeostasis mediated by AGC1 has been implicated in the pathogenesis of autistic spectrum disorder2.
Species reactivity key:
Anti-SLC25A12 Antibody (#ANT-112) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize AGC1 from human, mouse, and rat samples.