Anti-SLC25A12

AGC1, ARALAR1, Solute Carrier Family 25 Member 12, Mitochondrial Aspartate Glutamate Carrier 1, Calcium Binding Mitochondrial Carrier Superfamily Member Aralar1
    Cat #: ANT-112
    Sizes: 25 µl, 50 µl, 0.2 ml
  • Lyophilized Powder
  • Antigen Incl.
  • QC Tested
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)KDKSKSGMISGLD, corresponding to amino acid residues 169-181 of mouse SLC25A12 (Accession Q8BH59). Intracellular, N-terminus.
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, human – identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
    Western blot
    Western blot analysis of rat skeletal muscle lysate (lanes 1 and 4, 1:600), mouse brain membrane (lanes 2 and 5) and human OVCAR3 cell line lysate (lanes 3 and 6):
    1-3. Anti-SLC25A12 antibody (#ANT-112), (1:200).
    4-6. Anti-SLC25A12 antibody, preincubated with the control peptide antigen.
    References
    1. Palmieri, F. (2013) Mol. Aspects Med. 34, 465.
    2. Napolioni, V. et al. (2011) Mol. Neurobiol. 44, 83.
    Scientific background

    SLC25 is a large family of nuclear-encoded transporters embedded in the inner mitochondrial membrane and in a few cases other organelle membranes.

    SLC2512A is a gene the aspartate/glutamate carrier1 (AGC1). AGC1 possesses six transmembrane alpha helices and N- and C-termini on the cytosolic side of the membrane1.

    AGC1 catalyzes an exchange between intra-mitochondrial aspartate and cytosolic glutamate, an important step in urea synthesis. As a component of the malate–aspartate shuttle (MAS), AGC1 also has a role in the transfer of NADH, carrying reducing equivalents from the cytosol into the mitochondrial matrix. AGC1 is expressed predominantly in heart and skeletal muscle, with weaker expression in brain and kidney. AGC1 is the main AGC isoform present in the adult brain, and it is expressed mainly in neurons. As part of the MAS, AGC1 has a role in the physiology of pancreatic β-cells by influencing glucose-induced activation of mitochondrial metabolism and insulin secretion and it is the only AGC isoform expressed in pancreatic islets and β-cells. 

    AGC1 has several Ca2+-binding EF-hand motifs located on its long, hydrophilic amino-terminus which extends into the intermembrane space and its activity is strongly regulated by extra-mitochondrial Ca2+. Dysregulation of cellular Ca2+ homeostasis mediated by AGC1 has been implicated in the pathogenesis of autistic spectrum disorder2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 04/02/2018

    Anti-SLC25A12 (#ANT-112) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize AGC1 from human, mouse, and rat samples.

    For research purposes only, not for human use