Anti-S1PR1 (EDG1) (extracellular) Antibody

Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CD363, CHEDG1
    Cat #: ASR-011
    Alternative Name Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CD363, CHEDG1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide SDYVNYDIIVRHYN(C), corresponding to amino acid residues 17-30 of human S1PR1 (Accession P21453). Extracellular, N-terminus.
    • Anti-S1PR1 (EDG1) (extracellular) Antibody
    Accession (Uniprot) Number P21453
    Gene ID 1901
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat - 13/14 amino acid residues identical.
    RRID AB_2039836.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.75 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, lci, wb
    May also work in: ifc*, ip*
    Western blot
    • Anti-S1PR1 (EDG1) (extracellular) Antibody
      Western blot analysis of RAEC cells (lanes 1 and 3) and A-10 (lane 2 and 4) cell lysates:
      1,2. Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011), (1:200).
      3,4. Anti-S1PR1 (EDG1) (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-S1PR1 (EDG1) (extracellular) Antibody
      Expression of Sphingosine 1-phosphate receptor 1 in rat lung
      Immunohistochemical staining of paraffin embedded rat lung sections using Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011), (1:100). Staining is present in vascular smooth muscle (black arrows) but not in the muscular layer of bronchi (red arrows). Hematoxilin is used as the counterstain.
    Live cell imaging / Immunocytochemistry
    • Anti-S1PR1 (EDG1) (extracellular) Antibody
      Expression of Sphingosine 1-phosphate receptor 1 in mouse 3T3 cells
      Cell surface detection of Sphingosine 1-phosphate receptor 1 in mouse live 3T3 cells. A. Extracellular staining of cells using Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011), (1:50), (red). B. DAPI is used for nuclear staining (blue). Merged image of A and B.
    1. Pyne, S. and Pyne, N.J. (2000) Biochem. J. 349, 385.
    2. Hla, T. et al. (2001) Science 294, 1875.
    3. Takuwa, Y. et al. (2002) J. Biochem. 131, 767.
    4. Rosen, H. et al. (2009) Annu. Rev. Biochem. 78, 743.
    5. Rosen, H and Liao, J. (2003) Curr. Opin. Chem. Biol. 7, 461.
    Scientific background

    Lysophospholipids constitute a group of important lipid mediators; lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (S1P), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2. Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG); S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3.

    Sphingosine 1-phosphate receptor 1 (S1PR1) is ubiquitously expressed. S1PR1 mRNA is detected in the brain, heart, spleen, liver, lung, thymus, kidney, skeletal muscle and lymphoid tissues4. Knockout of the gene encoding S1PR1 is lethal and therefore makes in vivo studies difficult5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 23/03/2020

    Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize S1PR1 from human, rat, and mouse samples.

    For research purposes only, not for human use



    Scientific Background


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