Anti-STIM1 (extracellular) Antibody

Stromal interaction molecule 1, GOK
    Cat #: ACC-063
    Alternative Name Stromal interaction molecule 1, GOK
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide CHSEDEKLSFEAVR, corresponding to amino acid residues 56-69 of human STIM1 (Accession Q13586). STIM1 expressed on the plasma membrane: Extracellular, N-terminus. STIM1 expressed on the ER: Luminal, N-terminus.
    • Anti-STIM1 (extracellular) Antibody
    Accession (Uniprot) Number Q13586
    Gene ID 6786
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse, bovine, canis - identical.
    RRID AB_2039893.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.45 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 10 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ifc, ih, ip, lci, wb
    Western blot
    • Anti-STIM1 (extracellular) Antibody
      Western blot analysis of RBL (lanes 1 and 4), HL-60 (lanes 2 and 5), and Jurkat (lanes 3 and 6) cell lysates:
      1,2,3. Anti-STIM1 (extracellular) Antibody (#ACC-063), (1:1000).
      4,5,6. Anti-STIM1 (extracellular) Antibody, preincubated with the negative control antigen.
    Immunoprecipitation
    • Rat isolated cardiomyocytes (Saliba, Y. et al. (2015) Cardiovasc. Res. 105, 248.).
    Immunohistochemistry
    • Anti-STIM1 (extracellular) Antibody
      Expression of STIM1 in rat stomach
      Immunohistochemical staining of paraffin embedded rat stomach sections using Anti-STIM1 (extracellular) Antibody (#ACC-063), (1:100). STIM1 is expressed in the parietal cells of the gastric mucosa (arrows). Hematoxilin is used as the counterstain.
    Indirect flow cytometry
    • Anti-STIM1 (extracellular) Antibody
      Cell surface detection of STIM1 in intact RBL (A) and Jurkat (B) cells.
      ___ Cells + FITC-conjugated goat anti-rabbit antibody.
      ___ Cells + Anti-STIM1 (extracellular) Antibody (#ACC-063), (5-10 µg antibody/0.5-1x106 cells) + FITC-conjugated goat anti-rabbit antibody.
    • The negative control antigen is not suitable for this application.
    Live cell imaging / Immunocytochemistry
    • Anti-STIM1 (extracellular) Antibody
      Expression of STIM1 in RBL cells
      Cell surface detection of STIM1 in live rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells with Anti-STIM1 (extracellular) Antibody (#ACC-063), (1:50) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B. Nuclear staining of cells using the cell-permeable dye Hoechst 33342. C. Merged image of panels A and B.
    References
    1. Eisner, D.A. et al. (2005) Exp. Physiol. 90, 3.
    2. Chakrabarti, R. and Chakrabarti, R. (2006) J. Cell. Biochem. 99, 1503.
    3. Feske, S. et al. (2006) Nature 441, 179.
    4. Pickett, J. (2006) Nature Reviews Mol. Cell Biol. 7, 794.
    5. Dziadek, M.A. and Johnstone, L.S. (2007) Cell Calcium 42, 123.
    6. Yuan, J.P. et al. (2007) Nat. Cell Biol. 9, 636.
    7. Muik, M. et al. (2008) J. Biol. Chem. 283, 8014.
    8. Li, Z. et al (2007) J. Biol. Chem. 282, 29448.
    9. Hewavitharana, T. et al. (2007) Cell Calcium 42, 173.
    Scientific background

    Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, and cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become of great interest.

    Recently, several key players of the store-operated complex have been identified.The Orai family consists of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2. Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4 The majority of STIM1 appears to be localized intracellularly at the ER membrane while low expression of STIM1 has been detected on the cell surface of several cell types.5 STIM1 has an amino-terminal EF hand Ca2+-binding domain facing the lumen of the ER.6 Upon Ca2+ store depletion, STIM1 molecules are redistributed in punctae underneath the plasma membrane and activate SOCs.

    Several possible interactions between STIM1 and Orai1 have been suggested. The most simple and cited is a dynamic interaction between the cytosolic C-terminus of STIM1 and the cytoplasmic domain of the Orai1 channel.7-9 STIM1 is assumed to regulate the activity of all known SOCs, including native SOCs.Consistent with their important role as calcium sensors within the ER, STIM1 proteins are ubiquitously expressed.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 16/03/2020

    Anti-STIM1 (extracellular) Antibody (#ACC-063) is a highly specific antibody directed against an epitope of the human Stromal interaction molecule 1. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize STIM1 from human, rat, and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. Mouse NSC-34 motor neuron cell lysate (1:1000).
      Tedeschi Vet al. (2019) Sci Rep9, 10743.
    2. Rat lung lysates (1:1000).
      Zhang, W. et al. (2017) Inflammation 40, 778.
    3. Human HES2-CM cell lysate.
      Wang, Y. et al. (2015) Stem Cells 33, 2973.
    4. Rat isolated cardiomyocytes.
      Saliba, Y. et al. (2015) Cardiovasc. Res. 105, 248.
    5. Human primary coronary artery smooth cell lysate.
      Munoz, E. et al. (2013) Cell Calcium 54, 375.
    Immunoprecipitation citations
    1. Mouse Motor Neuron (NSC-34) Cell Line (1:200).
      Tedeschi Vet al. (2019) Sci Rep9, 10743.
    2. Rat isolated cardiomyocytes (20 μg).
      Saliba, Y. et al. (2015) Cardiovasc. Res. 105, 248.
    Immunohistochemistry citations
    1. Rat lung sections (1:100).
      Zhang, W. et al. (2017) Inflammation 40, 778.
    Immunocytochemistry citations
    1. Human HES2-Cm cells.
      Wang, Y. et al. (2015) Stem Cells 33, 2973.
    More product citations
    1. Steinckwich, N. et al. (2011) J. Immunol. 186, 2182.
    2. Bomben, V.C. and Sontheimer, H. (2010) Glia 58, 1145.
    3. Bréchard, S. et al. (2009) Biochem. Pharmacol. 78, 504.
    4. Ohba, T. et al. (2009) Biochem. Biophys. Res. Commun. 389, 172.
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