- Peptide CHSEDEKLSFEAVR, corresponding to amino acid residues 56-69 of human STIM1 (Accession Q13586). STIM1 expressed on the plasma membrane: Extracellular, N-terminus. STIM1 expressed on the ER: Luminal, N-terminus.
- Western blot analysis of rat RBL basophilic leukemia (lanes 1 and 4), human HL-60 promyelocytic leukemia (lanes 2 and 5), and human Jurkat T-cell leukemia (lanes 3 and 6) cell lysates:1,2,3. Anti-STIM1 (extracellular) Antibody (#ACC-063), (1:1000).
4,5,6. Anti-STIM1 (extracellular) Antibody, preincubated with STIM1 (extracellular) Blocking Peptide (#BLP-CC063).
- Rat isolated cardiomyocytes (Saliba, Y. et al. (2015) Cardiovasc. Res. 105, 248.).
- Expression of STIM1 in rat stomachImmunohistochemical staining of paraffin embedded rat stomach sections using Anti-STIM1 (extracellular) Antibody (#ACC-063), (1:100). STIM1 is expressed in the parietal cells of the gastric mucosa (arrows). Hematoxilin is used as the counterstain.
- Cell surface detection of STIM1 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-PE.
___ Cells + Anti-STIM1 (extracellular) Antibody (#ACC-063), (5μg) + goat-anti-rabbit-PE.
- The blocking peptide is not suitable for this application.
- Expression of STIM1 in RBL cellsCell surface detection of STIM1 in live rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells with Anti-STIM1 (extracellular) Antibody (#ACC-063), (1:50) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B. Nuclear staining of cells using the cell-permeable dye Hoechst 33342. C. Merged image of panels A and B.
- Eisner, D.A. et al. (2005) Exp. Physiol. 90, 3.
- Chakrabarti, R. and Chakrabarti, R. (2006) J. Cell. Biochem. 99, 1503.
- Feske, S. et al. (2006) Nature 441, 179.
- Pickett, J. (2006) Nature Reviews Mol. Cell Biol. 7, 794.
- Dziadek, M.A. and Johnstone, L.S. (2007) Cell Calcium 42, 123.
- Yuan, J.P. et al. (2007) Nat. Cell Biol. 9, 636.
- Muik, M. et al. (2008) J. Biol. Chem. 283, 8014.
- Li, Z. et al (2007) J. Biol. Chem. 282, 29448.
- Hewavitharana, T. et al. (2007) Cell Calcium 42, 173.
Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, and cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become of great interest.
Recently, several key players of the store-operated complex have been identified.3 The Orai family consists of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2. Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4 The majority of STIM1 appears to be localized intracellularly at the ER membrane while low expression of STIM1 has been detected on the cell surface of several cell types.5 STIM1 has an amino-terminal EF hand Ca2+-binding domain facing the lumen of the ER.6 Upon Ca2+ store depletion, STIM1 molecules are redistributed in punctae underneath the plasma membrane and activate SOCs.
Several possible interactions between STIM1 and Orai1 have been suggested. The most simple and cited is a dynamic interaction between the cytosolic C-terminus of STIM1 and the cytoplasmic domain of the Orai1 channel.7-9 STIM1 is assumed to regulate the activity of all known SOCs, including native SOCs.5 Consistent with their important role as calcium sensors within the ER, STIM1 proteins are ubiquitously expressed.
Species reactivity key:
Anti-STIM1 (extracellular) Antibody (#ACC-063) is a highly specific antibody directed against an epitope of the human Stromal interaction molecule 1. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize STIM1 from human, rat, and mouse samples.
- Western blot of mouse cardiomyocyte cells. Tested in cells transfected with STIM1 siRNA.
Zhu, J. et al. (2021) J. Inflamm. Res. 14, 3945.
- Mouse NSC-34 motor neuron cell lysate (1:1000).
Tedeschi V. et al. (2019) Sci Rep. 9, 10743.
- Rat lung lysates (1:1000).
Zhang, W. et al. (2017) Inflammation 40, 778.
- Human HES2-CM cell lysate.
Wang, Y. et al. (2015) Stem Cells 33, 2973.
- Rat isolated cardiomyocytes.
Saliba, Y. et al. (2015) Cardiovasc. Res. 105, 248.
- Human primary coronary artery smooth cell lysate.
Munoz, E. et al. (2013) Cell Calcium 54, 375.
- Human HES2-Cm cells.
Wang, Y. et al. (2015) Stem Cells 33, 2973.
- Mouse brain sections (1:250).
Kikuta, S. et al. (2019) Front. Cell. Neurosci. 13, 547.