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Anti-Thromboxane A2 Receptor (extracellular) Antibody

Prostanoid TP receptor, TXA2-R, TBXA2R

Cat #: APR-095
Alternative Name Prostanoid TP receptor, TXA2-R, TBXA2R
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RPTNITLEERRLIAS, corresponding to amino acid residues 13 - 27 of human TBXA2R (Accession P21731). Extracellular, N-terminus.
Accession (Uniprot) Number P21731
Gene ID 6915
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat – 12 out of 15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Applications: ifc, wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of rat spleen lysate (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and mouse brain lysate (lanes 3 and 6):
    Western blot analysis of rat spleen lysate (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and mouse brain lysate (lanes 3 and 6):
    1-3. Anti-Thromboxane A2 Receptor (extracellular) Antibody (#APR-095), (1:200).
    4-6. Anti-Thromboxane A2 Receptor (extracellular) Antibody, preincubated with Thromboxane A2 Receptor (extracellular) Blocking Peptide (BLP-PR095).
  • Western blot analysis of human K562 chronic myelogenous leukemia cell line lysate (lanes 1 and 4), human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 5) and human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 3 and 6):
    Western blot analysis of human K562 chronic myelogenous leukemia cell line lysate (lanes 1 and 4), human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 5) and human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 3 and 6):
    1-3. Anti-Thromboxane A2 Receptor (extracellular) Antibody (#APR-095), (1:200).
    4-6. Anti-Thromboxane A2 Receptor (extracellular) Antibody, preincubated with Thromboxane A2 Receptor (extracellular) Blocking Peptide (BLP-PR095).
Indirect flow cytometry
  • Cell surface detection of Thromboxane A2 Receptor by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    Cell surface detection of Thromboxane A2 Receptor by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti- Thromboxane A2 Receptor (extracellular) Antibody (#APR-095), (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Yan, A. et al. (2017) Mol. Med. Rep. 16, 2491.
  2. Zhao, Z. et al. (2017) Oncotarget 8, 30030.
  3. Kinsella, B.T. (2016) Wilms Tumor, Brisbane (AU): Codon Publications, Chapter 11.
Scientific background

Thromboxane A2 (TxA2) is a prostanoid that is synthesized from arachidonic acid and plays an important role in haemostasis, a process regulating platelet aggregation and vascular tone. Thromboxane A2 binds to the thromboxane A2 receptor (TBXA2R) to induce strong vasoconstriction in renal arteries.

Thromboxane A2 receptor is a seven-transmembrane G-protein coupled receptor localized on the cell membrane and in intracellular compartments. TBXA2R is expressed in the central nervous system in several types of cells, such as microglia, oligodendrocytes and astrocytes. Imbalances in the levels of the receptor is widely implicated in several cardiovascular, pulmonary, renal and prostate pathologies1-3.

Thromboxane A2 receptor mediates vasoconstriction and ischemia and its activation is implicated in atherosclerosis and inflammation. Activation of the receptor induces endothelial cell apoptosis by inhibiting Akt phosphorylation and also inhibits endothelial cell migration and angiogenesis by decreasing Akt and eNOS phosphorylation1-3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Last update: 11/04/2021

Anti-Thromboxane A2 Receptor (extracellular) Antibody (#APR-095) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize TBXA2R from mouse, rat and human samples.

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For research purposes only, not for human use
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