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Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody

Prostaglandin E2 receptor EP3 subtype, PGE2 receptor EP3 subtype, Prostanoid EP3 receptor

Cat #: APR-065
Alternative Name Prostaglandin E2 receptor EP3 subtype, PGE2 receptor EP3 subtype, Prostanoid EP3 receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RAPHWYASHMKTR, corresponding to amino acid residues 137-149 of mouse prostanoid EP3 receptor (Accession P34980). 2nd intracellular loop.
Accession (Uniprot) Number P34980
Gene ID 24929
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2756761.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of rat kidney (lanes 1 and 3) and pancreas (lanes 2 and 4) membranes:
    Western blot analysis of rat kidney (lanes 1 and 3) and pancreas (lanes 2 and 4) membranes:
    1,2. Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody (#APR-065), (1:200).
    3,4. Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody, preincubated with Prostaglandin E Receptor EP3/PTGER3 Blocking Peptide (#BLP-PR065).
  • Western blot analysis of mouse kidney membranes:
    Western blot analysis of mouse kidney membranes:
    1. Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody (#APR-065), (1:200).
    2. Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody, preincubated with Prostaglandin E Receptor EP3/PTGER3 Blocking Peptide (#BLP-PR065).
  • Western blot analysis of human Jurkat T-cell leukemia cell lysate:
    Western blot analysis of human Jurkat T-cell leukemia cell lysate:
    1. Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody (#APR-065), (1:400).
    2. Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody, preincubated with Prostaglandin E Receptor EP3/PTGER3 Blocking Peptide (#BLP-PR065).
Indirect flow cytometry
  • Human blood eosinophils (Durchschein, F. et al. (2019) Dig. Dis. Sci. 64, 2806.).
References
  1. Kalinski, P. (2012) J. Immunol. 188, 21.
  2. Natarajan, C. et al. (2013) Mol. Pharmacol. 83, 206.
  3. Lu, A. et al. (2015) J. Clin. Invest. 125, 1228.
  4. Von Der Emde, L. et al. (2014) Am. J. Cancer. Res. 4, 952.
Scientific background

Prostaglandin is a small molecule produced from arachidonic acid by the enzymes COX1, COX2 and PG synthase. PGE2 regulates various factors of inflammatory response, promoting local vasodilatation and attracting immune system cells to the site of the inflammation.

Prostanoid EP3 receptor, a member of the G-protein coupled receptor superfamily, is one of four PGE2 receptors (EP1-EP4) and binds PGE2 with a higher affinity than other receptors of its class. Unlike EP2 and EP4, EP3 is not coupled to Gs and lacks cAMP activating functions. In contrast, most of its splice variants couple with Gi and inhibit adenylate cyclase activity1. EP3 binds prostaglandin in a GTP dependent manner. One of the receptor’s extracellular domains, ECII, can alter GTP effect on agonist binding and thus the receptor’s downstream intracellular signaling. ECII causes receptor conformational changes2.

EP3 plays a role in pulmonary hypertension (PAH) pathophysiology. It is hypothesized that EP3 levels are upregulated in pulmonary arterial smooth muscle cells and in human distal pulmonary arteries in response to hypoxia. Blocking agents for the EP3 receptor may prove as a useful strategy in the treatment of PAH3.

EP3 is also involved in the cell growth, invasion and migration of many tumor types including bladder cancer. EP3 has been found to be significantly less expressed in muscular and non-muscular invasive bladder cells than in normal urothelial cells4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title: Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody
EP3 receptor expression increases in LPS-treated astrocytes.Western blot analysis of rat primary astrocyte using Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody (#APR-065). EP3 receptor expression increases following LPS stimulation (right lane).Adapted from Paniagua-Herranz, L. et al. (2017) Front. Pharmacol. 8, 937. with permission of Frontiers.
Last update: 11/04/2021

Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody (#APR-065) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize EP3 receptor from rat, mouse, and human samples.

For research purposes only, not for human use
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