- Peptide (C)EDQSN(S)TVPSYPA(S)RD, corresponding to amino acid residues 647-662 of rat TRPV4 (Accession Q9ERZ8). 3rd extracellular loop.
- Mouse J774 macrophage cells, human THP-1 monocytic leukemia cells (2.5 µg).
- Cell surface detection of TRPV4 in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + rabbit IgG isotype control-FITC.
___ Cells + Anti-TRPV4 (extracellular)-FITC Antibody (#ACC-124-F), (2.5 µg).
TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells1. According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP)1-4. The TRPV subfamily consists of six members, TRPV1-65.
TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland6.