Anti-TRPV2 (VRL1) (extracellular)-FITC Antibody

Transient receptor potential cation channel subfamily V member 2, Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Stretch-activated channel 2B, SAC2B
    Cat #: ACC-039-F
    Alternative Name Transient receptor potential cation channel subfamily V member 2, Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Stretch-activated channel 2B, SAC2B
  • Lyophilized Powder
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    Immunogen
    • Peptide (C)HQPSLDQPAIPSSKAT, corresponding to amino acid residues 413-428 of rat TRPV2 (Accession Q9WUD2). 1st extracellular loop.
    • Anti-TRPV2 (VRL1) (extracellular)-FITC Antibody
    Accession (Uniprot) Number Q9WUD2
    Gene ID 29465
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 15/16 amino acid residues identical.
    RRID AB_2040269.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-039), and direct flow cytometry (labeled antibody).
    Applications: fc
    Direct flow cytometry
    • Anti-TRPV2 (VRL1) (extracellular)-FITC Antibody
      Cell surface detection of TRPV2 in live intact mouse BV-2 microglia cells:
      ___ Cells.
      ___ Cells + rabbit IgG isotype control-FITC.
      ___ Cells + Anti-TRPV2 (VRL1) (extracellular)-FITC Antibody (#ACC-039-F), (5 µg).
    • Anti-TRPV2 (VRL1) (extracellular)-FITC Antibody
      Cell surface detection of TRPV2 in intact living RBL cells:
      ___ Unstained cells. 
      ___ Cells + Anti-TRPV2 (VRL1) (extracellular)-FITC Antibody, (#ACC-039-F).
    • The control antigen is not suitable for this application.
    References
    1. Montell, C. et al. (2002) Mol. Cell 9, 229.
    2. Clapham, D.E. (2003) Nature 426, 517.
    3. Moran, M.M. et al. (2004) Curr. Opin. Neurobiol. 14, 362.
    4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
    5. Gunthorpe, M.J. et al. (2002) Trends Pharmacol. Sci. 23, 183.
    6. Tominaga, M. and Caterina, M.J. et al. (2004) J. Neurobiol. 61, 3.
    7. Voets, T. et al. (2004) Nature 430, 748.
    8. Pedersen, S.F. et al. (2005) Cell Calcium 38, 233.
    9. Muraki, K. et al. (2003) Circ. Res. 93, 829.
    10. Shimosato, G. et al. (2005) Pain 119, 225.
    11. Saunders, C.I. et al. (2007) Mol. Immunol. 44, 1429.
    12. Caterina, M.J. et al. (1999) Nature 398, 436.
    Scientific background

    TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have six putative transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-termini of all TRPs are intracellular3.

    According to IUPHAR, the TRP family is comprised of numerous subfamilies on the basis of sequence homology; TRPC, TRPM, TRPV, TRPA, TRPML, and TRPP1-4. The TRPV subfamily consists of six members, TRPV1-65.

    Four members of the TRPV family have been described as thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (<17°C) to noxious heat (>52°C) 6,7. Although it shares around 50% homology with TRPV1, TRPV2 is not activated by capsaicin nor by protons. It has a high temperature threshold of ~52°C and is considered to play an essential role in the perception of high-intensity noxious heat stimulation8-10. TRPV2 is also considered to be a stretch-activated channel and to play a role in skeletal and cardiac muscle degeneration and pain pathway8. The TRPV2 channel is expressed in DRG neurons, different brain regions and non-neuronal tissues such as the spleen, lung and intestine and in components of the immune system5,11,12.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, live cell imaging, and indirect flow cytometry applications. It has been designed to recognize TRPV2 from mouse and rat samples.

    Anti-TRPV2 (VRL1) (extracellular)-FITC Antibody (#ACC-039-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used for immunofluorescent applications such as direct flow cytometry using live cells.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Shipping and Ordering information