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Anti-Human CaV1.2 (CACNA1C) Antibody

Human α1C
Cat #: ACC-022
Alternative Name Human α1C
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide VNENTRMYIPEENHQ(C), corresponding to amino acid residues 2-15 of human CaV1.2 (exon 1B) (Accession Q13936). Intracellular, N-terminus.
Accession (Uniprot) Number Q13936
Gene ID 775
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat - 14/15 amino acid residues identical.
RRID AB_2039769.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity The antibody recognizes mouse CaV1.2, splice variants P22002-4 and P22002-5 of rat CaV1.2 and splice variant P15381-4 of rabbit CaV1.2.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, wb
May also work in: ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat heart membranes:
    Western blot analysis of rat heart membranes:
    1. Anti-Human-CaV1.2 (CACNA1C) Antibody (#ACC-022), (1:200).
    2. Anti-Human-CaV1.2 (CACNA1C) Antibody, preincubated with Human Cav1.2/CACNA1C Blocking Peptide (#BLP-CC022).
  • Mouse cortex, hippocampus, and cerebellum lysates (1:200) (White, J.A. et al. (2008) Learn. Mem. 15, 1.).
Immunocytochemistry
References
  1. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579.
  2. IUPHAR
Scientific background

All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal, cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors.

While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous and CaV1.3 channels are found in the heart, brain and pancreas. Several peptidyl toxins are described that are specific L-type channel blockers, but so far no selective blocker for one of the CaV1 isoforms have been described. These include the Mamba toxins Calcicludine (#SPC-650), Calciseptine (#C-500) and FS-2 (#F-700).

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human CaV1.2 channel. Anti-Human CaV1.2 (CACNA1C) Antibody (#ACC-022) can be used in western blot and immunocytochemistry applications. It has been designed to recognize CaV1.2 from mouse, rat and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Mouse cortex, hippocampus, and cerebellum lyastes (1:200).
    White, J.A. et al. (2008) Learn. Mem. 15, 1.
Immunocytochemistry citations
  1. Rat nodose ganglion neurons in culture (1:15).
    Pamidimukkala, J. and Hay, M. (2004) Brain Res. 1006, 215.
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