Anti-CaV1.2 (CACNA1C)-ATTO-488 Antibody

Voltage-dependent L-type calcium channel subunit α1C
Cat #: ACC-003-AG
Alternative Name Voltage-dependent L-type calcium channel subunit α1C
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)TTKINMDDLQPSENEDKS, corresponding to amino acid residues 848-865 of rat CaV1.2 (Accession P22002). Intracellular loop between domains II and III.
    Accession (Uniprot) Number P22002
    Gene ID 24239
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; guinea pig - 17/18 amino acid residues identical; human, rabbit - 16/18 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-003), and immunohistochemistry (labeled antibody).
    Applications: ic, ih
      • Expression of CaV1.2 in rat cerebellum
        Immunohistochemical staining of rat cerebellum frozen sections with Anti-CaV1.2 (CACNA1C)-ATTO-488 Antibody (#ACC-003-AG), (1:100). Both dendrites of Purkinje cells (horizontal arrows) and fibers of Bergmann glia (vertical arrows) were stained. DAPI (blue Nissl counterstain) helps define the layers: granule (G), Purkinje (P) and molecular (M).
        Expression of CaV1.2 in rat pancreas
        Immunohistochemical staining of paraffin embedded sections of rat pancreas using Anti-CaV1.2 (CACNA1C)-ATTO-488 Antibody (#ACC-003-AG), (1:50). Staining is highly specific for endocrine cells of the Isle of Langerhans (IL).
      • Expression of CaV1.2 in mouse pancreatic microvascular endothelial cells (MS1)
        Immunocytochemical staining of MS1 cells. A. Intracellular staining of paraformaldehyde-fixed and permeabilized MS1 cells with Anti-CaV1.2 (CACNA1C)-ATTO-488 Antibody (#ACC-003-AG), (1:50). B. The cell-permeable dye Hoechst 33342 (blue) was used for nuclear staining. C. Merged image of panels A and B.
      • All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal, cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors.

        While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous and CaV1.3 channels are found in the heart, brain and pancreas. Several peptidyl toxins are described that are specific L-type channel blockers, but so far no selective blocker for one of the CaV1 isoforms have been described. These include the Mamba toxins Calcicludine (#SPC-650), Calciseptine (#C-500) and FS-2 (#F-700).

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 03/06/2019

    Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003) is a highly specific antibody directed against an epitope of rat CaV1.2 channel. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize CaV1.2 from mouse, rat, and human samples.

    Anti-CaV1.2 (CACNA1C)-ATTO-488 Antibody (#ACC-003-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-CaV1.2 (CACNA1C)-ATTO-488 Antibody has been tested in immunohistochemical and immunocytochemical applications and is specially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use
    Citations
      • Immunohistochemical staining of mouse brain sections with #ACC-003. Tested in CACNA1D-/- conditional knockout mice.
        Jeon, D. et al. (2010) Nat. Neurosci. 13, 482.
    Related Products
        • Guinea pig Anti-CaV1.2 (CACNA1C) Antibody (#AGP-001) is raised in guinea pig and can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit. This antibody has been raised against the same epitope as Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003).