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Anti-KCNE1 (IsK) Antibody

Delayed rectifier potassium channel subunit IsK, Minimal potassium channel, MinK
Cat #: APC-163
Alternative Name Delayed rectifier potassium channel subunit IsK, Minimal potassium channel, MinK
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)RSKKLEHSHDPFN, corresponding to amino acid residues 68-80 of rat KCNE1 (Accession P15383). Intracellular, C-terminus.
Accession (Uniprot) Number P15383
Gene ID 25471
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, pig, dog – identical; human – 12/13 amino acid residues identical.
RRID AB_2756750.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat kidney (lanes 1 and 4), rat heart (lanes 2 and 5) and mouse kidney (lanes 3 and 6) membranes:
    Western blot analysis of rat kidney (lanes 1 and 4), rat heart (lanes 2 and 5) and mouse kidney (lanes 3 and 6) membranes:
    1-3. Anti-KCNE1 (IsK) Antibody (#APC-163), (1:200).
    4-6. Anti-KCNE1 (IsK) Antibody, preincubated with KCNE1/IsK Blocking Peptide (#BLP-PC163).
References
  1. Charpentier, F. et al. (2010) J. Mol. Cell. Cardiol. 48, 37.
  2. Tristani-Firouzi, M. and Sanguinetti, M.C. (2003) J. Mol. Cell. Cardiol. 35, 27.
  3. Neyroud, N. et al. (1997) Nat. Genet. 15, 186.
  4. Chen, H.Y. et al. (2003) Science 299, 251.
Scientific background

Potassium delayed rectifier channels are a sub-type of potassium channels found in various species of animals. They are responsible for the late-repolarization phase and the duration of action potential, especially in cardiac myocytes.

There are two types of delayed rectifier channels (Ik). The Fast component is known as Ikr and is encoded by the gene KCNH2 in Xenopus. This channel’s α-subunit is a 6-transmembrane domains voltage dependent potassium channel and its regulatory β-subunit, MiRP1, is required to reconstitute native Ikr1.

KCNE1 and KCNQ1 are the genes encoding the two components of the Iks channel and this association was not fully understood for a long time. KCNQ1 (KV7.1) is a protein with six transmembrane domains and a typical pore loop containing a potassium signature sequence.

The KNCE1 gene encodes the protein “minK”. This gene is one of family of genes that encode small channel subunits (smaller than 130 amino-acids) that co-assemble with bigger subunits thus changing channel gating properties. These are currently the smallest known potassium channel subunits. Their small size and putative transmembrane domain prevent them from forming channels on their own.  In addition, in an experiment where mammalian cells were transfected with minK, no K+ currents were observed. When transfecting cells with a combination of KNCE1 and KNCQ1 the currents observed were up to ten times larger than currents induced by the KNCQ1 subunit alone2.

The loss of function of Iks is tightly linked with the long QT syndrome and bilateral deafness and gain of function was found to be linked with cardiac tachy-arrhythmias3,4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KCNE1. Anti-KCNE1 (IsK) Antibody (#APC-163) can be used in western blot analysis. It has been designed to recognize KCNE1 from rat, mouse and human samples.

For research purposes only, not for human use
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