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Hm1a Toxin

Delta-theraphotoxin-Hm1a, Delta-TRTX-Hm1a, Heteroscodratoxin-1, HmTx1, Kappa-theraphotoxin-Hm1a, Kappa-TRTX-Hm1a
A Selective and Potent Activator of NaV1.1 Channels and Blocker of KV2 and KV4 Channels
Cat #: STH-601
Alternative Name Delta-theraphotoxin-Hm1a, Delta-TRTX-Hm1a, Heteroscodratoxin-1, HmTx1, Kappa-theraphotoxin-Hm1a, Kappa-TRTX-Hm1a
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 3996.4 Da.
    Purity: >98% (HPLC)
    Form Lyophilized powder.
    Effective concentration 100 nM – 1 µM.
    Sequence ECRYLFGGCSSTSDCCKHLSCRSDWKYCAWDGTFS
    Modifications Disulfide bonds between Cys2-Cys16, Cys9-Cys21 and Cys15-Cys28. Ser35 - C-terminal amidation.
    Structure
    Molecular formula C170H240N48O53S6.
    Activity Hm1a is a NaV1.1 specific activator1.
    References-Activity
    1. Osteen, J.D. et al. (2016) Nature 534, 494.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs Hm1a Toxin enhances the current of NaV1.1 channels expressed in Xenopus oocytes.
      Alomone Labs Hm1a Toxin enhances the current of NaV1.1 channels expressed in Xenopus oocytes.
      A. Representative time course of Hm1a Toxin (#STH-601) effect on the normalized area of NaV1.1 channel current. Membrane potential was held at -90 mV, current was elicited by a 100 ms voltage step to -10 mV every 10 sec and was significantly enhanced by the application of 250 nM Hm1a Toxin (green). B. Superimposed traces of NaV1.1 current after application of control (black) and of 250 nM Hm1a Toxin (green), taken from the recording in A.
    References - Scientific background
    1. Escoubas, P. et al. (2002) Mol. Pharmacol. 62, 48.
    2. Osteen, J.D. et al. (2016) Nature 534, 494.
    3. Osteen, J.D. et al. (2017) Proc. Natl. Acad. Sci. U.S.A. 114, 6836.
    Scientific background

    Hm1a Toxin (δ-TRTX-Hm1a) is a peptide toxin originally isolated from Heteroscodra maculate tarantula venom. The toxin was originally described as a KV2 and KV4 channel blocker1 but has since been found to be a selective and specific activator of NaV1.1 voltage-gated sodium channels2,3. It interacts with extracellular loops connecting transmembrane segments 1-2 and 3-4 in domain IV voltage sensor of the channel to inhibit NaV1.1 fast inactivation2,3. Hm1a inhibits human NaV1.1 channel inactivation expressed in Xenopus oocytes with EC50 value of 38 ± 6 nM.

    NaV1.1 channel is a therapeutic target for brain disorders, such as epilepsy, Alzheimer's disease, and autism. It also contributes to mechanical pain by regulating excitability in a specific subset of sensory neurons within the peripheral nervous system.

    Target NaV1.1, KV2, KV4 channels
    Net Peptide Content: 100%
    Image & Title Hm1a Toxin
    Alomone Labs Hm1a Toxin enhances the current of NaV1.1 channels transiently expressed in HEK293 cells.Representative superimposed traces of NaV1.1 currents in the absence (black) or presence of 250 nM Hm1a Toxin (#STH-601), (green). Currents were elicited by a 20 ms voltage step to 0 mV every 10 sec from a holding potential of -120 mV.This figure was kindly provided by the lab of Dr. Moran Rubinstein, Goldschleger Eye Research Institute, The Department of Human Molecular Genetics and Biochemistry, Sackler School of Medicine, Tel Aviv University.
    Last update: 21/10/2020

    Hm1a Toxin (#STH-601) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Electrophysiology
    1. HEK-293 cells expressing NaV1.1 channel.
      Nissenkorn, A. et al. (2019) PLoS ONE 14, e0211901.
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