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Few examples in the literature emphasize the importance of using BDNF-biotin in living cells experiments. Pardridge, W.M. et al. demonstrated that the delivery of BDNF to the brain is non-existent owing to the combined effects of neglible blood brain barrier (BBB) transport and rapid systemic clearance1. The brain delivery of BDNF may be increased by conjugating biotinylated BDNF to BBB drug delivery vectors, such as neutral avidin conjugated to murine monoclonal antibody to the rat transferrin receptor1. Zhang, Y. and Pardridge, W.M. further showed that when BDNF is formulated to enable transport across the BBB, the intravenous administration of BDNF results in the reduction in stroke volume and improvement in functional outcome2.
Du, J. et al. detected by using BDNF-biotin the ligand-induced TrkB internalization in cultured hippocampal neurons3.
Bhattacharyya, A. et al. showed in mature sciatic nerves, that biotinylated BDNF activated Trk receptors function as rapid retrograde signal carriers to execute remote responses to target-derived neurotrophins4.
Song, X.Y. et al. proved that exogenous BDNF-biotin is transported by the peripheral nerves following injection into the rat footpad and can be found in the sciatic nerves in fibres and vesicles5. Their data suggest that peripherally applied BDNF may have therapeutic effects on injured spinal cord. Xie, W. et al. followed the trafficking of QD-BDNF (Quantum Dot-BDNF) after its internalization at the axon terminal6. Their result showed that QD-BDNF could be used to track the movement of exogenous BDNF in neurons over long distances and to study the signaling organelles that contain BDNF6.
TrkB binds to BDNF at the plasma membrane.Live TIRF imaging of TrkB-ACP-488 and labeled BDNF co-movement in HEK293T (HEK). Cells treated with streptavidin-647-labeled human BDNF-Biotin (#B-250-B) for 5 minutes and imaged at 8 fps. Arrowhead marks co-localized TrkB-BDNF particle. Scale bar = 2 µm. Right: color trace shows movement trajectory of TrkB-ACP-488 and BDNF-647 particles over time (blue- start, red- end of trajectory) Scale bar = 1 µm.Unpublished data from Zahavi, E.E. et al. (2018) Sci. Signal. 11, eaao4006. Figure generously provided by Dr. Eran Perlson, Dept. of Physiology and Pharmacology, Tel-Aviv University, Tel-Aviv, Israel.
human BDNF-Biotin (#B-250-B) is a highly pure, recombinant, and biologically active conjugated protein.
Zahavi, E.E. et al. (2018) Sci. Signal. 11, eaao4006.
- Recombinant human BDNF protein (#B-250)
- Recombinant human BDNF proDomain protein (#B-245)
- Recombinant human proBDNF (cleavage resistant) protein (#B-256)
- Recombinant human proBDNF protein (#B-257)
- Recombinant mouse proBDNF (cleavage resistant) protein (#B-243)
- Recombinant mouse proBDNF protein (#B-240)
- Anti-TrkB (extracellular) Antibody (#ANT-019)
- Anti-TrkB (extracellular)-ATTO-488 Antibody (#ANT-019-AG)
- Anti-TrkB (extracellular)-FITC Antibody (#ANT-019-F)
- AZD-1332 (#A-495)