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Certificate of Analysis

KCNN4 (KCa3.1) Channel Basic Research Pack

All You Need for KCNN4 (KCa3.1) Channel Research
Cat #: ESB-400 7 Vials

Antibodies

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-KCNN4 (KCa3.1, SK4) Antibody Cat #: APC-064

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide RQVRLKHRKLREQV(C), corresponding to amino acid residues 350-363 of rat KCNN4 (Accession Q9QYW1). Intracellular, C-terminal part.
Homology
Mouse, human, pig - identical.

Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody Cat #: ALM-051

Size: 100 µg Host: Mouse Type: Monoclonal Application: IC, IF, IFC, IH, IP, LCI, WB Reactivity: H, M, R
Immunogen
Synthetic peptide mapping to the 3rd extracellular loop of human KCNN4 (Accession O15554).

Pharmacological Reagents

1-EBIO Cat #: E-150

Size: 25 mg Source: Synthetic MW: 162.19 Purity: >97%
Effective concentration
5-100 μM.
Activity
1-EBIO is an activator of KCa3.1 and KCa2 channel currents1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
250 mM DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

SKA-31 Cat #: S-120

Size: 10 mg Source: Synthetic MW: 200.2 Purity: >98%
Effective concentration
0.1-10 µM.
Activity
SKA-31 is an activator of KCa3.1 and KCa2 channels (EC50 values are 260, 2900, 2900 nM for KCa3.1, KCa2.1 and KCa2.2 respectively)1. Potentiates acetylcholine-induced EDHF-type dilations of mouse carotid arteries and lowers blood pressure in normotensive and hypertensive mice1,2. SKA-31 blocks TRPM73.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

NS-6180 Cat #: N-230

Size: 10 mg Source: Synthetic MW: 323.3 Purity: >98%
Effective concentration
10 nM – 1 μM.
Activity
NS-6180 blocks SK4 channels with IC50 of  9, 14 and 15 nM for rat, human and mouse erythrocyte KCa3.1 channels respectively1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
100mM DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

TRAM 34 Cat #: T-105

Size: 10 mg Source: Synthetic MW: 344.8 Purity: >99%
Effective concentration
10 nM - 1 μM.
Activity
TRAM 34 is a potent and selective KCa3.1 (SK4, intermediate conductance Ca2+-activated K+) channel inhibitor1. As such, it is a powerful pharmacological tool that enables the determination of the roles played by KCa3.1 channels in different physiological systems ranging from activation of T lymphocytes to migration of endothelial cells2-3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.

Note
A mouse monoclonal antibody (#ALM-051) is included in this Research Pack. Please take into account when reacting with a secondary antibody.