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Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-ATTO-488 Antibody

IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel
    Cat #: ALM-051-AG
  • Lyophilized Powder
  • Shipped at Room Temp.
    • Type: Monoclonal
    Source: Mouse
    Reactivity: h, m, r
    Immunogen
    Synthetic peptide mapping to the 3rd extracellular loop of human KCNN4 (Accession O15554).
    Accession (Uniprot) Number O15554
    Gene ID 3783
    Purity Affinity purified from cultured hybridoma medium.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype IgM.
    Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
    Clone 6C1.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ALM-051), and immunocytochemistry (labeled antibody).
    Applications: fc, ic, lci
    May also work in: ih
    Direct flow cytometry

    Human CD8+ T cells (Chimote, A.A. et al. (2018) Sci. Signal. 11, 527.).

    Live cell imaging / Immunocytochemistry
    Expression of KCNN4 in live intact human LNCaP prostate carcinoma cells
    Immunocytochemical staining (green) of live intact human LNCaP prostate carcinoma cells with Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-ATTO-488 Antibody (#ALM-051-AG), (1:15) (A). B. Live view of the cells. C. Merge of the two images.
    References
    1. Ghanshani, S. et al. (2000) J. Biol. Chem. 275, 37137.
    2. Hoffman, J.F. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 7366.
    Scientific background

    KCNN4 (KCa3.1, SK4) is a member of the Ca2+ activated K+ channel family that shares the characteristic of being activated by intracellular Ca2+. The channel has an intermediate conductance, is voltage insensitive and is activated by Ca2+ in the submicromolar range. The channel has a similar topology to that of KV channels, that is, six transmembrane domains and intracellular N- and C-termini. 

    KCNN4 is widely expressed in epithelial, endothelial and cells of hematopoietic origin. In erythrocytes (red blood cells) it has been identified as the molecular correlate of the so-called Gardos channel.  

    The functional role of the channel is to set the cell membrane potential at negative values so as to aid in the electrochemical transport of other ions such as Cl- and Ca2+. Indeed, KCNN4 has a key role in sustaining the Ca2+ influx in activated T lymphocytes and in regulating Cl- secretion from colon epithelium. Therefore, specific blockers of the KCNN4 channel have been proposed for the treatment of several diseases including autoimmune diseases, secretory diarrhea and sickle cell anemia.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Lyophilized Powder
    Last update: 09/12/2018

    Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody (#ALM-051) is a highly specific monoclonal antibody directed against an epitope of the human channel. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize KCNN4 from human, rat, and mouse samples.

    Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-ATTO-488 Antibody (#ALM-051-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-ATTO-488 Antibody has been tested in live cell imaging applications and is specially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use
    Citations
    Direct flow cytometry citations

    1. Human CD8+ T cells.
      Chimote, A.A. et al. (2018) Sci. Signal. 11, 527.

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