Alomone Labs is pleased to offer the nAChR Agonist Explorer Kit (#EK-204). This Explorer Kit includes nAChR agonists, ideal for screening purposes.
Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs) which belong to the superfamily of G-protein coupled receptors (GPCRs) and nicotinic AChRs (nAChRs) which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name1.
To date, 17 different, but related, subunits of nAChRs have been identified and cloned. They consist of α subunits (α1-10), which are responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, 3 conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer of these various subunits organized around a central pore1.
nAChRs are validated therapeutic targets for various CNS pathologies4.
- nAChR Antagonist Explorer Kit (#EK-205)
- nAChR Modulator Explorer Kit (#EK-202)
- nAChR Antibody Explorer Kit (#AK-203)
- nAChRα Antibody Explorer Kit (#AK-231)
- nAChRβ Antibody Explorer Kit (#AK-232)
- nAChR Antibodies for Pain Research Explorer Kit (#AK-375)
- Muscle Specific nAChR Antagonist Explorer Kit (#EK-207)