Every lot is tried & tested in a relevant biological assay.
- Alomone Labs Neurotensin induces Ca2+ influx in HT29 cells.Cells were loaded with Fluo-3AM Ca2+ probe. Ca2+ influx was measured in the presence of increasing Neurotensin (#GPN-100) concentrations. The maximum Ca2+ levels is plotted for each neurotensin concentration (ED50 = 142 ng/ml).
- Kislauskis, E. et al. (1988) J. Biol. Chem. 263, 4963.
- Vincent, J.P. et al. (1999) Trends Pharmacol. Sci. 20, 302.
- Almenoff, J. et al. (1981) Biochem. Biophys. Res. Commun. 102, 206.
- McCann, S.M. and Vijayan, E. (1992) Annals of the New York Academy of Sciences. 668, 287.
- Binder, E.B. et al. (2001) Pharmacol. Rev. 53, 453.
- Maeno, H. et al. (2004) Brain Res. 998, 122.
Neurotensin is a peptide neurotransmitter hormone produced from a 170 amino acid precursor protein, first isolated in 1973 from bovine hypothalamus1. Neurotensin signaling is transmitted through three receptors, NTS1 and NTS2 are 7-transmembrane G-protein coupled receptors, and NTS3 is a type I membrane receptor with a large extra cellular domain2.
Neurotensin synaptic transmission is terminated primarily by specific enzymatic cleavage of neurotensin by several peptidases3. Neurotensin participates in the regulation of Hypophyseal Prolactin and Luteinizing Hormone (LH) secretion4 and has a pivotal role in the dopaminergic system regulation.
Neurotensin is implicated in body temperature regulation, feeding behavior, locomotion regulation5 and pain transmission6.
GPCR ligands are now part of our growing portfolio of GPCR related products, which also includes antibodies.
Neurotensin (#GPN-100) is a highly pure, synthetic, and biologically active peptide.