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Certificate of Analysis

NMDA Receptor 2B Basic Research Pack

All You Need for NMDA Receptor 2B Research

Cat #: ESB-750
Size: 4 Vials
Form: Lyophilized

Antibodies

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-NMDAR2B (GluN2B) (extracellular) Antibody Cat #: AGC-003

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)NTHEKRIYQSNMLNR, corresponding to amino acid residues 323-337 of rat NMDA receptor 2B (Accession Q00960). Extracellular, N-terminus.
Homology
Human, mouse - identical.

Pharmacological Reagents

NMDA Cat #: N-170

Size: 250 mg Source: Synthetic MW: 147.1 Purity: >98%
Effective concentration
6.5-500 µM.
Activity
Prototypic NMDA receptor agonist. An amino acid that, as a D-isomer, is the defining agonist for the NMDA receptor subtype of glutamate receptors1. NMDA is a water-soluble synthetic substance that is not normally found in biological tissues2. EC50 ranges between 6.5-25.0 μM, depending on the tissue applied on1,3,4.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Water. Centrifuge all product preparations before use (10000 x g for 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

5,7-Dichlorokynurenic acid Cat #: D-190

Size: 50 mg Source: Synthetic MW: 258.06 Purity: >98%
Effective concentration
0.5-20 µM.
Activity
5,7-Dichlorokynurenic acid is an NMDA receptor antagonist acting at the glycine site1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
200 mM in DMSO. Centrifuge all product preparations before use (10 000 X g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

(+)-MK 801 maleate Cat #: M-230

Size: 50 mg Source: Synthetic MW: 337.4 Purity: >99% (HPLC)
Effective concentration
0.01-10 µM.
Activity
(+)-MK 801 maleate is a potent, selective and non-competitive NMDA receptor antagonist with an IC50 of 0.01 μM1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Up to 100 mM in DMSO. Centrifuge all product preparations before use (5,000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.

For research purposes only, not for human use
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