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Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody

NMDA receptor 2B, Ionotropic glutamate receptor subunit ε2, N-methyl-D-aspartate receptor subunit 2B, GRIN2B, NR2B
Cat #: AGC-003-AR
Alternative Name NMDA receptor 2B, Ionotropic glutamate receptor subunit ε2, N-methyl-D-aspartate receptor subunit 2B, GRIN2B, NR2B
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)NTHEKRIYQSNMLNR, corresponding to amino acid residues 323-337 of rat NMDAR2B (Accession Q00960). Extracellular, N-terminus.
Accession (Uniprot) Number Q00960
Gene ID 24410
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human, mouse - identical.
RRID AB_2040027.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGC-003), and immunohistochemistry (labeled antibody).
Applications: if, ih
May also work in: ic*, lci*
Immunohistochemistry
  • Multiplex staining of GluN2A and GluN2B in mouse deep cerebellar nucleus
    Multiplex staining of GluN2A and GluN2B in mouse deep cerebellar nucleus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody (#AGC-003-AR), (1:60) and Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002), (1:200). A. Sections were incubated with Anti-NMDAR2A (GluN2A) (extracellular) Antibody, followed by goat anti-rabbit-Alexa-488 (green). B. The same sections were incubated with Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody (red). C. Merge of A and B demonstrates the ubiquitous colocalization of the GluN2A and GluN2B subunits in cells with neuronal profiles in this nucleus.  Arrows point at an example of NR2A and NR2B co-expression.
  • Expression of NMDAR2B (NR2B) in rat DRG
    Expression of NMDAR2B (NR2B) in rat DRG
    Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody (#AGC-003-AR), (red), (1:50). Staining is present in neuronal cell bodies. Hoechst 33342 is used as the counterstain (blue).
References
  1. Dingledine, R. et al. (1999) Pharmacol. Rev. 51, 7.
  2. Mayer, M.L. and Armstrong, N. (2004) Annu. Rev. Physiol. 66, 161.
  3. Prybylowski, K. and Wenthold, R.J. (2004) J. Biol. Chem. 279, 9673.
  4. Mayer, M.L. (2006) Nature 440, 456.
Scientific background

The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.

The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.

The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.

Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson’s and Alzheimer’s diseases.

NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title:

Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody
Multiplex staining of PSD-95 and GluN2B in rat brain
Immunohistochemical staining of perfusion-fixed frozen rat parietal cortex sections using Anti-PSD-95 Antibody (#APZ-009), (1:400) and Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody (#AGC-003-AR), (1:60). A. PSD-95 staining (green). B. The same sections were stained for GluN2B (red). C. Merge of the two images shows several cells expressing both proteins (arrows point to several examples). Nuclei are stained with DAPI (blue).

Last update: 16/08/2020

Anti-NMDAR2B (GluN2B) (extracellular) Antibody (#AGC-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, live cell imaging, immunohistochemistry, and immunoprecipitation applications. It has been designed to recognize GluN2B from human, rat, and mouse samples.

Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody (#AGC-003-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody has been tested in immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

For research purposes only, not for human use
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