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Certificate of Analysis

TRPM Channel Antibody Explorer Kit

A Screening Package of TRPM Channel Antibodies Economically Priced

Cat #: AK-210
Size: 8 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-Human TRPM1 Antibody Cat #: ACC-041

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H
Immunogen
Peptide (C)EEKKVKKEKASTETE, corresponding to amino acid residues 1518-1533 of human TRPM1 (Accession O75560). Intracellular, C-terminus.
Homology
Canis - 12/16 amino acid residues identical.

Anti-TRPM2 Antibody Cat #: ACC-043

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)HTFQGKEWDPKKHVQE, corresponding to amino acid residues 105-120 of mouse TRPM2 (Accession Q91YD4). Intracellular, N-terminus.
Homology
Rat - 15/16 amino acid residues identical; human - 14/16 amino acid residues identical.

Anti-TRPM3 (extracellular) Antibody Cat #: ACC-050

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide KNKDDMPYMSQAQEIH(C), corresponding to amino acid residues 816-831 of human TRPM3 (Accession Q9HCF6). 1st extracellular loop.
Homology
Rat, mouse - 15/16 amino acid residues identical.

Anti-TRPM4 Antibody Cat #: ACC-044

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide EKEQSWIPKIFKK(C), corresponding to amino acid residues 5-17 of human TRPM4 (Accession Q8TD43). Intracellular, N-terminus.
Homology
Rat, mouse - 12/13 amino acid residues identical.

Anti-TRPM5 Antibody Cat #: ACC-045

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide GSGKKRGKFVKVPS(C), corresponding to amino acid residues 32-45 of mouse TRPM5 (Accession Q9JJH7). Intracellular, N-terminus.
Homology
Rat, human - 13/14 amino acid residues identical.

Anti-TRPM6 (extracellular) Antibody Cat #: ACC-046

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide CVKDYDLERGPDEK, corresponding to amino acid residues 802-815 of mouse TRPM6 (Accession Q8CIR4). 1st extracellular loop.
Homology
Rat - 13/14 amino acid residues identical; human - 11/14 amino acid residues identical.

Anti-TRPM7 Antibody Cat #: ACC-047

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide CKRRKKDKTSDGPKLFLTEE, corresponding to amino acid residues 1146-1165 of human TRPM7 (Accession Q96QT4). Intracellular, C-terminus.
Homology
Rat, mouse - identical.

Anti-TRPM8 (extracellular) Antibody Cat #: ACC-049

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, LCI, N, WB Reactivity: H, M, R
Immunogen
Peptide SDVDGTTYDFAHC, corresponding to amino acid residues 917-929 of human TRPM8 (Accession Q7Z2W7). 3rd extracellular loop.
Homology
Canis - identical; bovine - 12/13 amino acid residues identical; rat, mouse - 11/13 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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