TRPM3 (extracellular) Blocking Peptide (#BLP-CC050) is the original antigen used for immunization during Anti-TRPM3 (extracellular) Antibody (#ACC-050) generation. The blocking peptide binds and ‘blocks’ Anti-TRPM3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membrane (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and mouse brain lysate (lanes 3 and 6):
- Mouse optic nerves sections (Papanikolaou, M. et al. (2017) Brain Struct. Funct. 222, 2993.).
- Expression of TRPM3 in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-TRPM3 (extracellular) Antibody (#ACC-050), (1:1200), followed by goat anti-rabbit-Alexa-488. A. TRPM3 immunoreactivity (green) appears in the purkinje cell layer (P, arrows) and in the molecular layer (MOL). B. Pre-incubation of the antibody with TRPM3 (extracellular) Blocking Peptide (#BLP-CC050), suppressed staining. Cell nuclei are stained with DAPI (blue).
- KNKDDMPYMSQAQEIH(C), corresponding to amino acid residues 816-831 of human TRPM3 (Accession Q9HCF6).