TRPM6 (extracellular) Blocking Peptide (#BLP-CC046) is the original antigen used for immunization during Anti-TRPM6 (extracellular) Antibody (#ACC-046) generation. The blocking peptide binds and ‘blocks’ Anti-TRPM6 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat kidney (lanes 1 and 4), rat testis (lanes 2 and 5) and mouse kidney (lanes 3 and 6) lysates:
- Mouse placenta sections (1:50) (Suzuki, Y. et al. (2016) J. Physiol. Sci. 67, 151.).
- Expression of TRPM6 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-TRPM6 (extracellular) Antibody (#ACC-046), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal CA1 region showed TRPM6 immunoreactivity (green) in in the pyramidal cell layer (P) and apical dendrites (arrows). B. Pre-incubation of the antibody with TRPM6 (extracellular) Blocking Peptide (BLP-CC046), suppressed staining. Cell nuclei are stained with DAPI (blue).
- CVKDYDLERGPDEK, corresponding to amino acid residues 802-815 of mouse TRPM6 (Accession Q8CIR4).