Anti-CHRM5 (extracellular) Antibody

Muscarinic acetylcholine receptor M5, Cholinergic receptor muscarinic 5, mAChR M5, HM5
    Cat #: AMR-005
    Alternative Name Muscarinic acetylcholine receptor M5, Cholinergic receptor muscarinic 5, mAChR M5, HM5
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, r
    May also work in: m*
      • Peptide (C)HNATTVNGTPVNHQPLER, corresponding to amino acid residues 7-24 of human M5 muscarinic receptor (Accession P08912). Extracellular, N-terminus.
        Anti-CHRM5 (extracellular) Antibody
    Accession (Uniprot) Number P08912
    Gene ID 1133
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 16/18 amino acid residues identical; rat - 14/18 amino acid residues identical.
    RRID AB_10658757.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*, lci*
      • Anti-CHRM5 (extracellular) Antibody
        Western blot analysis of rat lung (lanes 1 and 5) and brain (lanes 2 and 6) membranes, A-875 (lanes 3 and 7) and MALM-3M (lanes 4 and 8) cell lysates:
        1-4. Anti-CHRM5 (extracellular) Antibody (#AMR-005), (1:200).
        5-8. Anti-CHRM5 (extracellular) Antibody, preincubated with the negative control antigen.
    References
      • Acetylcholine is a neurotransmitter which activates two different groups of receptors: nicotinic acetylcholine receptors (nAChR) which belong to the superfamily of ligand-gated ion channels, and muscarinic acetylcholine receptors (mAChR) which belong to the G-protein coupled receptor (GPCR) superfamily. There are five separate gene products (M1-M5) that, assembled, make up this GPCR subfamily and like all GPCRs, have seven transmembrane spanning domains.

        Generally, M1M3 and M5 muscarinic receptors are known to activate phospholipase C (PLC) via Gq coupling, while M2 and M4 muscarinic receptors couple to Gi/o, and therefore inhibit adenylate cyclase1. This classification is however not clear cut.

        mAChR can activate adenylate cyclase by coupling to Gs2. In addition, M2 and M4 muscarinic receptors, when overexpressed, can also activate adenylate cyclase in some systems3-5.

        Evidence suggests that muscarinic receptors can form homo- or heterodimers and that the dimer formed can subsequently affect the downstream signaling pathways1.

        Muscarinic receptors have been shown to regulate voltage-gated Ca2+ channels, namely CaV2.16, as well acid sensing ion channels (ASIC)7. Generally speaking, the actions of muscarinic receptors on ion channels can be either via 2nd messengers or through their direct action on the channels once activated8.

        Furthermore, muscarinic receptors can also promote endocytosis of ion channels (KV1.2 for example) by recruiting tyrosine kinases that phosphorylate the channel in order to terminate its activity9. Other diverse and important functions of muscarinic receptors include cell growth, survival and physiology1.

        Expression of muscarinic receptors is found in neurons in the central nervous system as well as in the peripheral nervous system. These receptors are also expressed in cardiac and smooth muscle, lung, intestine, ovary and urothelium1,10.

        The M5 muscarinic receptor has been the most difficult receptor to study as there is no specific pharmacology targeted against the receptor and no high level of expression has been detected in tissues. However, the effects of single knockout mice were studied and it was observed that in M5 knockouts there is a moderate decrease in ligand binding in the lung and bladder, two organs expressing the M5 muscarinic receptor11. In addition, it has been proposed that the M5 muscarinic receptor may be involved in interstitial cystitis, a urinary bladder disorder12.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 12/09/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the human M5 muscarinic receptor. Anti-CHRM5 (extracellular) Antibody (#AMR-005) can be used for western blot analysis. It was specifically designed to recognize rat, mouse and human M5.

    For research purposes only, not for human use