µ-Conotoxin BuIIIB

Mu-Conotoxin BuIIIB
A Blocker of NaV1.4 and NaV1.3 and NaV1.2 Na+ Channels
    Cat #: STC-661
    Alternative Name Mu-Conotoxin BuIIIB
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 2764 Da.
    Purity: >98% (HPLC)
    Form Lyophilized powder.
    Effective concentration 1-20 nM.
    Sequence VGERCCKNGKRGCGRWCRDHSRCC.
    Modifications Disulfide bonds between Cys5-Cys17, Cys6-Cys23, and Cys13-Cys24. Cys24 – C-terminal amidation.
      • µ-Conotoxin BuIIIB
    Molecular formula C106H172N46O30S6.
    Activity µ-Conotoxin BuIIIB is a selective NaV1.2, NaV1.3 and NaV1.4 blocker1.
      • Wilson, M.J. et al.(2011) Proc. Natl. Acad. Sci. U.S.A108, 10302.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
      • µ-Conotoxin BuIIIB
        Alomone Labs µ-Conotoxin BuIIIB inhibits NaV1.4 currents heterologously expressed in Xenopus oocytes.
        A. Time course of µ-Conotoxin BuIIIB (#STC-661) blocking action on NaV1.4 peak current amplitude. Maximum current amplitudes were plotted as a function of time. Membrane potential was held at -100 mV and cells were stimulated by a 100 ms voltage step to -20 mV. 20 nM µ-Conotoxin BuIIIB were perfused as indicated by the bar (green) during 150 sec. B. Superimposed examples of NaV1.4 channel current in the absence (control) and presence (green) of 20 nM µ-Conotoxin BuIIIB (taken from the experiment in A).
    References - Scientific background
      • μ-Conotoxin BuIIIB, is a peptide toxin originally isolated from Conus Bullatus (Bubble cone). It is characterized by a six-cysteine frame-work (-CC-C-C-CC-) cross-linked by three disulfide bridges. μ-Conotoxin BuIIIB was demonstrated to be a potent irreversible inhibitor of the NaV skeletal muscle subtype NaV1.4 heterologously expressed in Xenopus oocytes. 1 μM toxin was shown to block 96% of the channel1. In addition, μ-conotoxin BuIIIB potently blocks NaV1.3 expressed in oocytes, with an IC50 of 0.1 μM. NaV1.3 is involved in pain perception; therefore μ-conotoxin BuIIIB should serve as a valuable tool to evaluate the contribution of the NaV1.3 subtype to various pain states2,3.
    Target NaV1.2, NaV1.3 and NaV1.4 Na+ channels
    Net Peptide Content: 100%
    Last update: 24/01/2020

    µ-Conotoxin BuIIIB (#STC-661) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use