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µ-Conotoxin BuIIIB

Mu-Conotoxin BuIIIB
A Blocker of NaV1.4 and NaV1.3 and NaV1.2 Na+ Channels
Cat #: STC-661
Alternative Name Mu-Conotoxin BuIIIB
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 2764 Da.
    Purity: >98% (HPLC)
    Form Lyophilized powder.
    Effective concentration 1-20 nM.
    Sequence VGERCCKNGKRGCGRWCRDHSRCC.
    Modifications Disulfide bonds between Cys5-Cys17, Cys6-Cys23, and Cys13-Cys24. Cys24 – C-terminal amidation.
    Structure
    Molecular formula C106H172N46O30S6.
    Activity µ-Conotoxin BuIIIB is a selective NaV1.2, NaV1.3 and NaV1.4 blocker1.
    References-Activity
    1. Wilson, M.J. et al.(2011) Proc. Natl. Acad. Sci. U.S.A108, 10302.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs µ-Conotoxin BuIIIB inhibits NaV1.4 currents heterologously expressed in Xenopus oocytes.
      Alomone Labs µ-Conotoxin BuIIIB inhibits NaV1.4 currents heterologously expressed in Xenopus oocytes.
      A. Time course of µ-Conotoxin BuIIIB (#STC-661) blocking action on NaV1.4 peak current amplitude. Maximum current amplitudes were plotted as a function of time. Membrane potential was held at -100 mV and cells were stimulated by a 100 ms voltage step to -20 mV. 20 nM µ-Conotoxin BuIIIB were perfused as indicated by the bar (green) during 150 sec. B. Superimposed examples of NaV1.4 channel current in the absence (control) and presence (green) of 20 nM µ-Conotoxin BuIIIB (taken from the experiment in A).
    References - Scientific background
    1. Holford, M. et al. (2009) Toxicon 53, 90.
    2. Norton, R.S. (2010) Molecules 15, 2825.
    3. Wilson, M.J. et al.(2011) Proc. Natl. Acad. Sci. U.S.A108, 10302.
    Scientific background μ-Conotoxin BuIIIB, is a peptide toxin originally isolated from Conus Bullatus (Bubble cone). It is characterized by a six-cysteine frame-work (-CC-C-C-CC-) cross-linked by three disulfide bridges. μ-Conotoxin BuIIIB was demonstrated to be a potent irreversible inhibitor of the NaV skeletal muscle subtype NaV1.4 heterologously expressed in Xenopus oocytes. 1 μM toxin was shown to block 96% of the channel1. In addition, μ-conotoxin BuIIIB potently blocks NaV1.3 expressed in oocytes, with an IC50 of 0.1 μM. NaV1.3 is involved in pain perception; therefore μ-conotoxin BuIIIB should serve as a valuable tool to evaluate the contribution of the NaV1.3 subtype to various pain states2,3.
    Target NaV1.2, NaV1.3 and NaV1.4 Na+ channels
    Net Peptide Content: 100%
    Last update: 23/08/2020

    µ-Conotoxin BuIIIB (#STC-661) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use
    Shipping and Ordering information