Anti-SCN3A (NaV1.3) Antibody

BIII, Brain type III Na+ channel, Sodium channel protein type 3 subunit alpha
    Cat #: ASC-004
    Alternative Name BIII, Brain type III Na+ channel, Sodium channel protein type 3 subunit alpha
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)HLEGNHRADGDRFP, corresponding to amino acid residues 511-524 rat NaV1.3 (Accession P08104). Intracellular, loop between domains I and II.
    • Anti-SCN3A (NaV1.3) Antibody
    Accession (Uniprot) Number P08104
    Gene ID 497770
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 12/14 amino acid residues identical.
    RRID AB_2040007.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 0.75 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, ip, wb
    May also work in: ifc*
    Western blot
    • Anti-SCN3A (NaV1.3) Antibody
      Western blot analysis of rat newborn brain membranes:
      1. Anti-SCN3A (NaV1.3) Antibody (#ASC-004), (1:200).
      2. Anti-SCN3A (NaV1.3) Antibody, preincubated with the negative control antigen.
    • Mouse membrane lysate (Kim, D.Y. et al. (2011) J. Biol. Chem. 286, 8106.).
    Immunoprecipitation
    • CNahIII-12 cells expressing human NaV1.3 and β1 subunit. (Meadows, L.S. et al. (2002) J. Neurosci. 22, 10669.).
    Immunohistochemistry
    • Anti-SCN3A (NaV1.3) Antibody
      Expression of NaV1.3 in rat embryo DRG
      Immunohistochemical staining of rat embryo dorsal root ganglion (DRG) frozen sections using Anti-SCN3A (NaV1.3) Antibody (#ASC-004), (1:100). NaV1.3 is expressed in DRG embryonic cells (arrows). Calibration bar = 50 µm.
    Immunocytochemistry
    • Anti-SCN3A (NaV1.3) Antibody
      Expression of NaV1.3 in rat DRG primary cells
      Immunocytochemical staining of paraformaldehyde-fixed and permeabilized  rat dorsal root ganglia (DRG) primary culture. A. DRG cells were stained using Anti-SCN3A (NaV1.3) Antibody (#ASC-004), (1:200) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B. Nuclear staining of cells using the cell-permeable dye Hoechst 33342. C. Merged image of panels A and B.
    References
    1. Wu, L. et al. (2002) NeuroReport 13, 2547.
    2. Fang, X. et al. (2002) J. Neurosci. 22, 7425.
    3. Fjell, J. et al. (2000) NeuroReport 11, 199.
    4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27.
    5. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
    6. Isom, L.L. (2001) Neuroscientist 7, 42.
    7. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 575.
    8. Lai, J. et al. (2004) Annu. Rev. Pharmacol. Toxicol. 44, 371.
    9. Hains, B.C. et al. (2003) J. Neurosci. 23, 8881.
    10. Cummins, T.R. et al. (2001) J. Neurosci. 21, 5952.
    11. Shah, B.S. et al. (2001) J. Physiol. 534.3, 763.
    Scientific background

    Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5  The NaV channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3

    Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7

    NaV1.3, also known as SCN3A, is highly expressed in embryonic sensory neurons and CNS, but its level dramatically decreases in adult rodents.8 Up-regulation of NaV1.3 channel expression was described in injured neurons and injured spinal cord.9-11

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-SCN3A (Nav1.3) Antibody NaV1.1 Levels Decrease in PV Cells of hAPP Mice and in AD Brains.A and B. Western blot analysis of parietal cortex from mice and inferior parietal cortex from humans using Anti-SCN1A (NaV1.1) Antibody (#ASC-001), Anti-SCN2A (NaV1.2) Antibody (#ASC-002), Anti-SCN3A (NaV1.3) Antibody (#ASC-004) and Anti-NaV1.6 (SCN8A) Antibody (#ASC-009). The graphs represent the quantitation of each western blot.Adapted from Verret, L. et al. (2012) with permission of Elsevier.
    Last update: 24/01/2020

    Anti-SCN3A (NaV1.3) Antibody (#ASC-004) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, and immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.3 from rat, human, and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Published figures using this product
    • Anti-SCN3A (NaV1.3) Antibody
      Expression of NaV1.6 increases in rat mECs during epileptogenesis.
      A. Immunohistochemical of rat mECs using Anti-NaV1.6 (SCN8A) Antibody (#ASC-009). NaV1.6 staining is detected in AIS and increases in post-SE tissue. The channel co-localizes with Ankryn-G, a marker of AIS. B. Ratio of post-SE and control tissues shows that NaV1.6 expression increases by 46%. C. Somatal expression for NaV1.6 and NaV1.2 (using Anti-SCN2A (NaV1.2) Antibody (#ASC-002)). D. Normalized expression of the channel expression shows that both NaV1.2 and NaV1.6 expression increases in the soma of post-SE tissues. NaV1.1 and NaV1.3 expression, detected using Anti-SCN1A (NaV1.1) Antibody (#ASC-001) and Anti-SCN3A (NaV1.3) Antibody (#ASC-004), respectively, does not change during epileptogenesis.
      Adapted from Hargus, N.J. et al. (2013) with permission of the American Physiological Society.
    Western blot citations
    1. Human astrocytoma lysate.
      Guan, G. et al. (2018) Neurosci. Lett. 674, 148.
    2. Mouse brain lysate.
      Lamar, T. et al. (2017) Neurobiol. Dis. 102, 38.
    3. Rat brain neurolemma lysate.
      Murenzi, E. et al. (2017) Neurotoxicology 60, 260.
    4. Rat trigeminal ganglion lysate.
      Yang, K.Y. et al. (2016) J. Dent. Res. 95, 1183.
    5. Rat pituitary (GH3) cell lysate.
      Baroni, D. et al. (2014) Biol. Cell 106, 13.
    6. Rat DRG lysate (1:200).
      Cheng, K.I. et al. (2014) Eur. J. Pain 18, 162.
    7. Rat DRG lysate (1:200).
      Shen, K.F. et al. (2013) Exp. Neurol. 247, 466.
    8. Mouse brain.
      Verret, L. et al. (2012) Cell 149, 708.
    9. Mouse brain membrane lysate.
      Kim, D.Y. et al. (2011) J. Biol. Chem. 286, 8106.
    Immunoprecipitation citations
    1. CNahIII-12 cells expressing human NaV1.3 and β1 subunit.
      Meadows, L.S. et al. (2002) J. Neurosci. 22, 10669.
    Immunohistochemistry citations
    1. Human brain sections.
      Guan, G. et al. (2018) Neurosci. Lett. 674, 148.
    2. Rat lumbar spinal cord sections.
      Wolff, M. et al. (2016) Neurosci. Res. 109, 16.
    3. Rat DRGs.
      Cheng, K.I. et al. (2014) Eur. J. Pain 18, 162.
    4. Rat brain sections (1:250).
      Hargus, N.J. et al. (2013) J. Neurophysiol. 110, 1144.
    5. Rat brain sections (1:200).
      Lindia, J.A. and Abbadie, C. (2003) Brain Res. 960, 132.
    Immunocytochemistry citations
    1. Mouse ventricular myocytes.
      Koleske, M. et al. (2018) J. Gen. Physiol. 150, 991.
    2. Rat DRGs (1:200).
      Shen, K.F. et al. (2013) Exp. Neurol. 247, 466.
    3. Mouse LVA myocytes (1:100).
      Ednie, A.R. et al. (2013) J. Mol. Cell. Cardiol. 59, 117.
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