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ω-Conotoxin CVIF

A Blocker of N-Type CaV Channels 

Cat #: STC-770
Lyophilized Powder yes
  • Bioassay Tested
    • Origin Synthetic peptide
    MW: 2665 Da
    Purity: >99% (HPLC)
    Effective concentration 50-100 nM.
    Sequence CKGKGASCRRTSYDCCTGSCRLGRC.
    Modifications Disulfide bonds between Cys1-Cys16, Cys8-Cys20 and Cys15-Cys27. Cys27 - C-terminal amidation.
    Structure
    Molecular formula C101H170N40O33S6.
    Activity ω-Conotoxin CVIF is an N-type Ca2+ channel blocker1.
    References-Activity
    1. Berecki, G. et al. (2010) Mol. Pharmacol. 77, 139.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs ω-Conotoxin CVIF inhibits CaV2.2 channel currents expressed in Xenopus oocytes.
      Alomone Labs ω-Conotoxin CVIF inhibits CaV2.2 channel currents expressed in Xenopus oocytes.
      A. Time course of ω-Conotoxin CVIF (#STC-770) action on maximum CaV2.2 channel (α1B + α2δ1 + β1) current elicited in 2 mM Ba2+. Peak current amplitudes were plotted as a function of time. Membrane potential was held at -100 mV and oocytes were stimulated by a 100 ms voltage step to +40 mV. 50 nM ω-Conotoxin CVIF were perfused as indicated by the bar (green) for 2.5 min. B. Superimposed examples of CaV2.2 channel maximum peak current in the absence (control) and presence (green) of 50 nM ω-Conotoxin CVIF (taken from the experiment in A).
    References - Scientific background
    1. Berecki, G. et al. (2010) Mol. Pharmacol. 77, 139.
    2. Sadeghi, M. et al. (2013) Mol. Pain 9, 51.
    Scientific background

    ω-Conotoxin CVIF is a peptide toxin originally isolated from Conus catus venom. It selectively blocks voltage-gated N-type Ca2+ channel and is being tested as a potential drug for the treatment of chronic and neuropathic pain. CVIF has a higher affinity for Ca2+ channels in their inactivated state.

    Neuronal (N)-type voltage-gated Ca2+channels (VGCCs) are involved in regulating neuronal excitability and nociceptive signals and play an important role in the transduction of acute and chronic pain perception. The VGCC channel residues that directly interact with ω-conotoxins, act at or near the outer vestibule of N-type (CaV2.2) and P/Q-type (CaV2.1) VGCCs1.

    CVIF can prevent the conduction of nociceptive signals from the peripheral to the central nervous system by inhibiting neurotransmitter release from primary afferent nerve terminals in the dorsal horn of the spinal cord1,2.

    CVIF has the ability to potently and selectively inhibit depolarization-activated Ba2+ currents through recombinant N-type Ca2+ channels in snails’ oocytes1.

    Target CaV2.2 channels
    Peptide Content: 100%
    Lyophilized Powder
    Last update: 02/01/2022

    ω-Conotoxin CVIF (#STC-770) is a highly pure, synthetic, and biologically active peptide toxin.

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    For research purposes only, not for human use
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