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[Ala1,3,11,15] Endothelin-1

A Potent Agonist of Endothelin B Receptors 
    Cat #: SPE-240
  • Lyophilized Powder
  • Bioassay Tested
    • Origin Synthetic peptide
    MW: 2368 Da.
    Purity: ≥98% (HPLC)
    Effective concentration 1 pM -100 nM.
    Sequence ASASSLMDKEAVYFAHLDIIW.
      • [Ala1,3,11,15] Endothelin-1
    Molecular formula C109H163N25O32S.
    CAS No.: 121204-87-3.
    Activity [Ala1,3,11,15 ] Endothelin-1 is a potent ET-B receptor agonist1.
      • Nakamichi, K. et al. (1992) Biochem. Biophys. Res. Commun. 182, 144.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Soluble in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
      • [Ala1,3,11,15] Endothelin-1
        Alomone Labs Ala[1,3,11,15] Endothelin-1 activates Endothelin B receptor-mediated Ca2+ mobilization in CHO-K1-mt Aequorin cells.
        Dose-response curve of normalized activation of the Endothelin B receptor-mediated Ca2+ mobilization, evoked by  Ala[1,3,11,15] Endothelin-1 (#SPE-240). Ca2+ response was detected by measuring aequorin-derived fluorescence following application of Ala[1,3,11,15] Endothelin-1 and Endothelin-1 at different concentrations. The EC50 measured for Ala[1,3,11,15] Endothelin-1 is 0.38 pM.
    References - Scientific background

      • [Ala1,3,11,15 ] Endothelin-1 is a potent ET-B (endothelin B) receptor agonist, a receptor that belongs to the G-protein coupled receptor (GPCR) superfamily. [Ala1,3,11,15] Endothelin-1 is a linear synthetic peptide analog of endothelin 1 (ET-1) that shows very high affinity for the receptor. The analog includes a substitution of Ala residue for Cys residues that removes the disulfide bridges that exist in endothelin-11-3.

        Endothelin-1 (ET-1) and its receptor ET-B play an important role in regulating renal function and blood pressure. ET-B Receptor and ET-1 are both expressed in sensory nerves. They are mainly present in medium- and large-sized cell bodies of human trigeminal ganglia1.

        Studies have shown that acute administration of an ET-B receptor agonist into the rat renal medulla leads to natriuresis and diuresis via a mechanism that is NOS1-dependent3.

    Target ET-B receptor
    Net Peptide Content: 100%
    Lyophilized Powder
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    Last update: 27/01/2020

    [Ala1,3,11,15] Endothelin-1 (#SPE-240) is a highly pure, synthetic, and biologically active peptide.

    For research purposes only, not for human use
    Citations
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