Certificate of Analysis

AMPA Receptor 2 Basic Research Pack

All You Need for AMPA Receptor 2 Research
Cat #: ESB-705 7 Vials

Antibody Compounds

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-GluR2 (GluA2) (extracellular) Antibody Cat #: AGC-005

Size: 0.2 ml Source: Rabbit Type: Polyclonal Application: IC, IH, IP, LCI, WB Reactivity: M, R
Immunogen
Peptide NVGNINNDKKDETYR(C), corresponding to amino acid residues 179-193 of rat GluR2 (Accession P19491). Extracellular, N-terminus.
Homology
Mouse - identical; human - 14/15 amino acid residues identical.

Non-Antibody Compounds

(RS)-AMPA hydrobromide Cat #: A-266

Size: 10 mg Source: Synthetic MW: 267.07 Purity: >95%
Effective concentration
0.1-100 µM.
Activity
(RS)-AMPA is a selective AMPA receptor agonist1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
50 mM in H2O. Centrifuge all product preparations before use (10,000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

CX 546 Cat #: C-210

Size: 25 mg Source: Synthetic MW: 247.3 Purity: >99%
Effective concentration
10-630 µM.
Activity
CX 546 is a selective AMPA receptor agonist1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
126 mM in DMSO. Centrifuge all product preparations before use (10000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

DNQX disodium salt Cat #: D-130

Size: 50 mg Source: Synthetic MW: 296.1 Purity: >99%
Effective concentration
0.25-500 µM.
Activity
AMPA/Kinate receptor antagonists. Association of AMPA receptors with TARPs converts DNQX from antagonists to weak partial agonists1. The concentrations that inhibit 50% of AMPA binding (IC50) for DNQX is 0.50 ± 0.10 µM, and about one-fifth as effective at kainate binding sites, 2.0 ± 0.1 uM in rat cortical membranes2. The IC50 of DNQX is 1 µM in a crystal structure of the GluR2 ligand binding core3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Water. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Naspm trihydrochloride Cat #: N-215

Size: 25 mg Source: Synthetic MW: 479.91 Purity: >95% (HPLC)
Effective concentration
300 nM - 10 µM.
Activity
Naspm trihydrochloride is a selective AMPA receptor antagonist; ~0.3 uM IC50 for AMPA induced currents in rat hipocampal neurons1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
50 mM in H2O. Centrifuge all product preparations before use (10000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

PhTx-74 Cat #: P-120

Size: 25 mg Source: Synthetic MW: 507.54 Purity: >99% (HPLC)
Effective concentration
0.1-100 μM.
Activity
PhTx-74 (Philanthotoxin-7,4) is a synthetic polyamine analogue of the naturally occurring wasp venom toxin philanthotoxin-4,3,3. It is a potent and selective antagonist of certain isoforms of the AMPA type of ionotropic glutamate receptors1. PhTx-74 inhibited GluR1 and GluR3 homomers as well as GluR1/GluR2 where 100 μM inhibited nearly 100% of the glutamate evoked currents. However, up to 500 μM had no or little effect on GluR2 or GluR2/GluR3 channels1-2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Water. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to one week at 4°C or six months at -20°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.