AMPA Receptor 2 Premium Research Pack

All You Need for AMPA Receptor 2 Research
  • Lyophilized Powder
  • Antigen Incl.
  • Bioassay Tested
  • Shipped at Room Temp.
Cat #: ESP-705
Sizes: 12 Vials
Last update: 04/09/2018

AMPA Receptor 2 Premium Research Pack (#ESP-705) contains all you need for GluA2 receptor research: AMPA receptor 2-specific antibodies and AMPA receptor pharmacological tools, all in one budget friendly package!

For research purposes only, not for human use
Compounds
Product NameCat #Size
Anti-GluR2 (GluA2) (extracellular) Antibody
AGC-005 1 x 0.2 ml
Guinea pig Anti-GluR2 (GluA2) (extracellular) Antibody
AGP-073 1 x 0.2 ml
(RS)-AMPA hydrobromide
A-266 1 x 10 mg
CX 546
C-210 1 x 25 mg
DNQX disodium salt
D-130 1 x 50 mg
Naspm trihydrochloride
N-215 1 x 25 mg
PD-161989 2-hydroxyethanesulfonate
P-285 1 x 10 mg
PEPA
P-200 1 x 25 mg
PhTx-74
P-120 1 x 25 mg
YM 90K hydrochloride
Y-105 1 x 10 mg
Note A guinea pig polyclonal antibody (#AGP-073) is included in this Research Pack. Please take into account when reacting with a secondary antibody.
Scientific Background
Scientific Background

AMPA receptors are members of the glutamate receptor family of ion channels that also include the NMDA and Kainate receptors. The three subfamilies are named after the original synthetic agonists that were identified as selective ligands of each family.

The α-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) receptor subfamily includes four members AMPA1-AMPA4 that are also known as GluR1-GluR4 respectively.

The functional AMPA channel is believed to be a tetramer, with most neuronal AMPA receptors actually being heterotetramers composed of AMPA1 plus AMPA2 or AMPA2 plus AMPA3, although homotetramers can also be found.

AMPA receptors are permeable to cations Na+ and K+ and Ca2+.  The Ca2+ permeability is dependent on the presence of AMPA2. The Ca2+ permeability of the AMPA2 subunit is determined by the presence of an arginine (R) at a critical site in the pore loop instead of a glutamine (Q) present in the same site in the other AMPA subunits. A post-transcriptional process known as RNA editing determines the presence of this R. Since most AMPA2 subunits in the adult brain have undergone RNA editing and most AMPA receptors contain the AMPA2 subunit, most native AMPA receptors will be impermeable to Ca2+.

Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that will open only in the presence of both glutamate and a depolarized membrane.

Synaptic strength, defined as the level of post-synaptic depolarization, can be long term (hence the term long term potentiation, LTP) and therefore induce changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning.

Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has focused in understanding the mechanisms that govern AMPA receptor trafficking.

References
  1. Dingledine, R. et al. (1999) Pharmacol. Rev51, 7.
  2. Sheng, M. and Lee, S.H. (2001) Cell 105, 825.
  3. Song, I. and Huganir, R.L. (2002) Trends Neurosci25, 578.
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