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Guinea pig Anti-BDNF Antibody

Brain-derived neurotrophic factor
Cat #: AGP-021
Alternative Name Brain-derived neurotrophic factor
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea pig
Reactivity: h, m, r
Immunogen
  • Peptide (C)VLEKVPVSKQLK, corresponding to amino acid residues 166-178 of human BDNF (precursor) (Accession P23560).
Accession (Uniprot) Number P23560
Gene ID 627
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat and many other species - identical.
RRID AB_2744680.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG.
Specificity The antibody is specific for BDNF; it does not crossreact with the closely related neurotrophins NGF, NT-3 or NT-4.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: cbe*, ic*, ip*
Western blot
Immunohistochemistry
  • Expression of BDNF in mouse hippocampus
    Expression of BDNF in mouse hippocampus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-BDNF Antibody (#AGP-021), (1:400), followed by goat anti-guinea pig-Cy3. BDNF staining (red) in the hippocampal dentate gyrus region, appears in neuronal profiles in the granule layer (G) and in interneurons in the hilus (H) region (arrows). DAPI counterstain is used to visualize nuclei of all cells (blue).
References
  1. Chao, M.V. (2003) Nat. Rev. Neurosci. 4, 299.
  2. Blum, R. and Konnerth, A. (2005) Physiology (Bethesda). 20, 70.
  3. Kalb, R. (2005) Trends Neurosci. 28, 5.
  4. Lu, B. et al. (2005) Nature Rev. Neurosci. 6, 603.
Scientific background

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors which includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).

All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.

BDNF conveys its activity by binding to two classes of receptors, a member of the Trk receptor tyrosine kinase family (TrkB) and the pan-neurotrophin receptor p75NTR. Binding of BDNF to the TrkB receptor triggers ligand-induced dimerization and autophosphorylation of tyrosine residues. This activates various signaling cascades such the MAPK, PI3K and PLCγ pathways that are involved in cell growth, survival and differentiation of neurons in the central and peripheral nervous system.

Interestingly, recent evidence suggests that BDNF may influence target cell function via ion channel modulation. Ion channel activity in the target cells can be modulated by a TrkB-mediated mechanism that has not yet been determined. BDNF is able to block both KV1.3 and AMPA-subtype glutamate ion channel currents in sensory neurons, while it can induce activation of the TRPC3 cation channel in neurons and of the NaV1.9 Na+ channel in hippocampal neurons. These newly recognized BDNF actions underlie its “rapid” neuronal functions that include changes in neuronal excitability, plasticity and synaptic transmission.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title: Guinea pig Anti-BDNF Antibody
Multiplex staining of BDNF and proBDNF in rat hippocampus.Immunohistochemical staining of rat hippocampal dentate gyrus perfusion-fixed frozen sections using Guinea pig Anti-BDNF Antibody (#AGP-021), (1:300) and Anti-proBDNF Antibody (#ANT-006), (1:200). A. BDNF staining (red) appears in interneuron outlines (arrows) in the hilus (H) region and in the granule layer (G). B. proBDNF staining (green) in the same section appears in interneuron outlines (arrows) in the hilus (H) region and in the granule layer (G). C. Merge of panel A and panel B shows extensive co-localization of the two proteins.
Last update: 23/08/2020

Guinea pig Anti-BDNF Antibody (#AGP-021) is a highly specific antibody directed against an epitope of human brain-derived neurotrophic factor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize BDNF from rat, human and mouse samples. The antibody is specific for BDNF; it does not crossreact with NGF, NT-3 or NT-4. The antigen used to immunize guinea pigs is the same as Anti-BDNF Antibody (#ANT-010) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

For research purposes only, not for human use
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