Anti-Calcium Sensing Receptor (extracellular) Antibody

CaSR, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, PCaR1, GPRC2A
    Cat #: ACR-004
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)DDYG RPGIE KFREE, corresponding to amino acid residues 216-229 of human CaSR (Accession P41180). N-terminus, extracellular.
    Accession (Uniprot) Number P41180
    Gene ID 846
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse, bovine, canis - identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.75 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ifc, ih, lci, wb
    May also work in: ip
    Western blot
    Western blot analysis of Colo205 (lanes 1 and 3), rat liver (lanes 2 and 4) and mouse kidney (lanes 5 and 6) lysates:
    1,2,5. Anti-Calcium Sensing Receptor (extracellular) Antibody (#ACR-004), (1:200).
    3,4,6. Anti-Calcium Sensing Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of CaSR in rat brain
    Immunohistochemical staining of rat hippocampal CA1 region using Anti-Calcium Sensing Receptor (extracellular) Antibody (#ACR-004). A. CaSR staining appears in the soma and appical dendrites of CA1 pyramidal neurons (arrows). B. Nuclear staining using DAPI as the counterstain. C. Merge image of A and B pannels
    Indirect flow cytometry
    Indirect flow cytometry analysis of live Jurkat (human acute T cell leukemia) cells:
    ___ Unstained cells.
    ___ Cells + Anti-Calcium Sensing Receptor (extracellular) Antibody (#ACR-004), (10 µg/5x105 cells).
    Live cell imaging / Immunocytochemistry
    Expression of CaSR in rat C6 cells
    Immunocytochemical staining of live intact rat glioma C6 cells. A. Extracellular staining of cells using Anti-Calcium Sensing Receptor (extracellular) Antibody (#ACR-004), (1:50), followed by Alexa-555-conjugated goat-anti-rabbit secondary antibody. B. Nuclear fluorescence staining of cells using Hoechst 33342. C. Merged images of panels A and B.
    References
    1. IUPHAR RECEPTOR DATABASE.
    2. Racz, G.Z. et al. (2002) Gut 51, 705.
    3. Canaff, L. and Hendy, G.N. (2002) J. Biol. Chem277, 30337.
    4. Chen, R.A. and Goodman, W.G. (2004) Am. J. Physiol. Renal Physiol286, F1005
    5. Chattopadhyay, N. (2006) Am. J. Physiol. Endocrinol. Metab290, E761.
    Scientific background

    The Calcium-sensing receptor (CaSR) is a member of subfamily C of the G-protein-coupled receptor (GPCR) superfamily. As its primary role, the CaSR is a key player in calcium homeostasis and is expressed in tissues involved in calcium metabolism such as parathyroid cells and the kidneys.1,2

    The expression of CaSR was also described in other cell types and tissues, such as neurons, keratinocytes, and the pancreas. However, the role of CaSR in these cells remains to be established.1,2 Following the identification of functional vitamin D response elements in the CaSR gene, it was suggested that vitamin D might regulate the expression of CaSR.3

    CaSR activation is followed by a rapid, transient increase in the cytosolic calcium concentration, resulting from mobilization of calcium from thapsigargin-sensitive intracellular stores, as well as an increased calcium influx through voltage-insensitive calcium channels located at the cell membrane.4

    CaSR regulates cellular processes such as proliferation, apoptosis, and differentiation under both normal and pathologic conditions.5

    Recent studies demonstrated the expression in human colon epithelium of CaSR, which regulates proliferation and differentiation. In colon carcinomas, lower levels of expression were found in the cancerous tissue compared to normal colon tissue.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 27/11/2018

    Anti-Calcium Sensing Receptor (extracellular) Antibody (#ACR-004) is a highly specific antibody directed against an epitope of the human CaSR. The antibody can be used in western blot, immunohistochemistry, indirect flow cytometry, and immunocytochemistry, and live cell imaging applications. It has been designed to recognize CaSR from human, rat, and mouse samples.

    For research purposes only, not for human use