Anti-Calnexin-ATTO-594 Antibody

Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90
  • New
Cat #: ACS-009-AR
Alternative Name Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)KAEEDEILNRSPRNRK, corresponding to amino acid residues 572-587 of rat Canx (Accession P35565). Intracellular, C-terminus.
    Accession (Uniprot) Number P35565
    Gene ID 29144
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 – 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACS-009), and immunohistochemical staining (labeled antibody).
    Applications: ih
    May also work in: ic
      • Immuno-colocalization of Calnexin and Presenilin-1 in mouse cortex
        Immunohistochemical staining of perfusion-fixed frozen mouse parietal cortex sections using Anti-Presenilin-1-ATTO-488 Antibody (#AIP-011-AG), (1:60) and Anti-Calnexin-ATTO-594 Antibody (#ACS-009-AR), (1:60). A. Calnexin staining (red) appears in neuronal profiles. B. Presenilin-1 staining (green) in the same section appears in neuronal profiles and apical dendrites (arrows). C. Merger of A and B demonstrates colocalization in several neurons (arrows). Cell nuclei are stained using DAPI (blue) as the counterstain. Images were acquired with 25x objective.
        Immuno-colocalization of Calnexin and Presenilin-1 in mouse hippocampus
        Immunohistochemical staining of perfusion-fixed frozen mouse hippocampal sections using Anti-Presenilin-1-ATTO-488 Antibody (#AIP-011-AG), (1:60) and Anti-Calnexin-ATTO-594 Antibody (#ACS-009-AR), (1:60). A. Calnexin staining (red) appears in neuronal profiles. B. Presenilin-1 staining (green) in the same section appears in neuronal profiles and apical dendrites (arrows). C. Merger of A and B demonstrates colocalization in several neurons (arrows). Cell nuclei are stained using DAPI (blue) as the counterstain. Images were acquired with 25x objective.
      • Calnexin is a type I transmembrane endoplasmic reticulum chaperone protein involved in the folding and assembly of numerous proteins. Calnexin is comprised of 537 amino acids with a large luminal domain consisting of 462 amino acids. Calnexin does not possess N-linked glycosylation sites and is localized to the endoplasmic reticulum by a –RKPRRE motif at its carboxyl terminus. Calnexin is one of the few ER membrane proteins that can be phosphorylated in a GTP-dependent manner1.

        Calnexin interacts with a number of ER membrane channels. It interacts transiently with wild-type Shaker K+ channel in the ER but fails to associate with an unglycosylated Shaker mutant that makes active, cell surface channels. This suggest that glycosylation of shaker protein is required for interaction with calnexin but calnexin is not required for the proper assembly and folding of Shaker channels2.

        Coexpression of Calnexin with the voltage-gated potassium channel KV1.2 was found to produce a substantial dose-dependent increase in cell surface KV1.2 channels in transfected mammalian COS-1 cells. In contrast, Calnexin coexpression showed no effect on trafficking of intracellularly retained KV1.1 or KV1.6 subunits3.

        Calnexin has been implicated in the pathophysiology of cystic fibrosis disease (CF). Wild type and ΔF508 mutant cystic-fibrosis conductance regulator (CFTR) associate with Calnexin in a Chinese hamster ovary (CHO) cell model. Although both wild-type and ΔF508 CFTRs are present in complexes with Calnexin, only wild-type CFTR is able to escape this association and exit the ER. This suggests that Calnexin retains misfolded proteins in the ER and contributes to the mislocalization of mutant CFTRs.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

    Check out more of our new products.

    Last update: 04/06/2019

    Anti-Calnexin Antibody (#ACS-009) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Canx from human, mouse, and rat samples.

    Anti-Calnexin-ATTO-594 Antibody (#ACS-009-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Calnexin-ATTO-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use