Anti-KV1.2 (KCNA2) (extracellular) Antibody

Potassium voltage-gated channel subfamily A member 2, RBK2
    Cat #: APC-162
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    May also work in: h
    Immunogen
    Peptide (C)RDENEDMHGGGVT, corresponding to amino acid residues 189-201 of rat KV1.2 (Accession P63142). 1st extracellular loop.
    Accession (Uniprot) Number P63142
    Gene ID 25468
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 12/13 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, lci, wb
    May also work in: ifc, ih, ip
    Western blot
    Western blot analysis of rat heart membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat brain membranes (lanes 3 and 6):
    1-3. Anti-KV1.2 (KCNA2) (extracellular) Antibody (#APC-162), (1:200).
    4-6. Anti-KV1.2 (KCNA2) (extracellular) Antibody, preincubated with the control peptide antigen.
    Live cell imaging / Immunocytochemistry
    Expression of KV1.2 in live intact rat PC12 cells
    Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-KV1.2 (KCNA2) (extracellular) Antibody (#APC-162), (1:50), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Live view of the cells. C. Merge of A and B.
    References
    1. McKinnon, D. (1989) J.Biol. Chem. 264, 8230.
    2. Gutman, G.A. et al. (2005) Pharmacol. Rev57, 473.
    3. Long, S.B. et al. (2005) Science 309, 897.
    4. Bogin, O. (2006) Modulator 21, 28.
    Scientific background

    KV1.2 is a mammalian voltage-dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.2 was first cloned from rat brain.1 Eight Shaker-related genes exist in mammals constituting the KV1, subfamily of the large KV channel family of genes.2

    A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function.

    The structure of KV1.2 channel is similar to all KV channels and includes six membrane spanning helices creating a voltage sensor domain and a pore domain.2

    The channel is expressed in neurons and cardiac and smooth muscle tissue as well as in retina and pancreas.2 The crystal structure of KV1.2 was recently solved shading light on the structure of a mammalian voltage dependent channel.3 The functional channel is considered low voltage activated and shows very little inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle.

    KV1.2 channels are sensitive to high doses of TEA (560 mM) and low doses of 4-AP (0.59 mM), the “classical” non-selective potassium channel blockers.

    Several venomous toxins from snakes, scorpions and honey bee are potent blockers (affecting the channels in the nanomolar range) of KV1.2 channels. Among these, the most potent and selective are α-Dendrotoxin (#D-350, 1-12 nM), Dendrotoxin-I (#D-390, 0.13 nM), Maurotoxin (STM-340, 0.1-0.8 nM), Hongotoxin-1 (#RTH-400, 0.17 nM), Margatoxin (#STM-325, 0.16-0.65 nM), Tityustoxin Kα (#STT-360, 0.21 nM) and MCD peptide (STM-250, 10-400 nM).4

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 25/11/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KV1.2. Anti-KV1.2 (KCNA2) (extracellular) Antibody (#APC-162) can be used in western blot and live cell imaging applications. It has been designed to recognize KV1.2 from human, rat and mouse samples.

    For research purposes only, not for human use
    Citations
    Related Products
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      • Antibodies for live cell imaging experiments
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          A fluorescent labeled specific KV1.2 blocker. It can be used in living cells to detect KV1.2 channels.