Anti-Synapsin I (SYN1) Antibody

Synapsin-1
    Cat #: ANR-014
    Alternative Name Synapsin-1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)RLHKKLGTEEFP(G), corresponding to amino acid residues 234 - 245 of rat Synapsin-1 (Accession P09951). Intracellular, region C.
        Anti-Synapsin I (SYN1) Antibody
    Accession (Uniprot) Number P09951
    Gene ID 24949
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    RRID AB_2756684.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
      • Anti-Synapsin I (SYN1) Antibody
        Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain synaptosomal fractions:
        1,2. Anti-Synapsin I (SYN1) Antibody (#ANR-014), (1:400).
        3,4. Anti-Synapsin I (SYN1) Antibody, preincubated with the negative control antigen.
      • Anti-Synapsin I (SYN1) Antibody
        Expression of Synapsin-1 in mouse cerebellum
        Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Synapsin I (SYN1) Antibody (#ANR-014), (1:400), followed by anti-rabbit-Alexa-488 antibody. Synapsin-1 staining (green) appears in Purkinje cell bodies (horizontal arrows) and in segments of the dendritic tree (vertical arrows). Nuclei are stained with DAPI (blue).
    References
      • Synapsins are a family of synaptic vesicle associated phosphoproteins consisting of three members: SYN1, SYN2, and SYN3, with several alternative splicing leading to 10 different isoforms. They are involved in synaptic transmission and plasticity, several stages of neurodevelopment, such as axon outgrowth and synapse formation. Synapsins are localized in presynaptic nerve terminals of central neurons1.

        Synapsin I is a peripheral membrane protein of synaptic vesicles that is phosphorylated at least at three different sites by various protein kinases. All synapsins have a single phosphorylation site in the short N-terminal domain A (site 1) that regulates neurite elongation and synaptic vesicle mobilization2.

        Synapsin I exhibits an asymmetric structure and exists in two forms, designated synapsin Ia and synapsin Ib. The primary structure of synapsin I is highly conserved, 95% of the protein’s residues are invariant and contain two major structural domains. The protein is divided into a hydrophobic, collagenase resistant head region and a proline rich, collagenase sensitive tail3,4

        Synapsin I has been suggested to play an important role in the regulation of neurotransmitter release and the organization of cytoskeletal organization including actin filaments, microtubules and spectrin in the presynaptic terminal5.

        In vitro studies show that the dephospho-form of synapsin I binds synaptic vesicles, binds and bundles F-actin, and promotes G actin polymerization5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Synapsin I. Anti-Synapsin I (SYN1) Antibody (#ANR-014) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Synapsin I from rat, mouse and human samples.

    For research purposes only, not for human use