Anti-KV1.2 (KCNA2) Antibody

Potassium voltage-gated channel subfamily A member 2, RBK2
    Cat #: APC-010
    Alternative Name Potassium voltage-gated channel subfamily A member 2, RBK2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • GST fusion protein with the sequence YHRETEGEEQAQYLQVTSCPKIPSSPDLKK SRSASTISKSDYMEIQEGVNNSNEDFREENLKTANCTLANTNYVNITKMLTDV, corresponding to amino acid residues 417-499 of rat KV1.2 (Accession P63142). Intracellular, C-terminus.
        Anti-KV1.2 (KCNA2) Antibody
    Accession (Uniprot) Number P63142
    Gene ID 25468
    Peptide confirmation Confirmed by DNA sequence and SDS-PAGE.
    Homology Mouse, dog, human, - identical.
    RRID AB_2313792.
    Purity The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 μl PBS.
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 3 µg fusion protein per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
      • Anti-KV1.2 (KCNA2) Antibody
        Western blot analysis of rat brain membranes:
        1. Anti-KV1.2 (KCNA2) Antibody (#APC-010), (1:200).
        2. Anti-KV1.2 (KCNA2) Antibody, preincubated with the negative control antigen.
        Anti-KV1.2 (KCNA2) Antibody
        Western blot analysis of rat heart membranes:
        1. Anti-KV1.2 (KCNA2) Antibody (#APC-010), (1:200)
        2. Anti-KV1.2 (KCNA2) Antibody, preincubated with the negative control antigen.
      • Anti-KV1.2 (KCNA2) Antibody
        Immuno-colocalization of KV1.2 and KV1.1 in mouse cerebellum
        Immunohistochemical staining of mouse perfusion-fixed frozen brain sections using Anti-KV1.2 (KCNA2) Antibody (#APC-010), (1:300) and Anti-KV1.1 (KCNA1) (extracellular)-ATTO-594 Antibody (#APC-161-AR), (1:100). A. KV1.2 staining, followed by donkey-anti-rabbit-Cy2 (green). B. KV1.1 staining (red). C. Merge of the two images suggests considerable co-localization in the pinceau structures (up-pointing arrows). KV1.1 also appears in blood vessels (down-pointing arrows), where no KV1.2 expression is observed.
        Mouse cerebellum (1:350) (Kleopa, K.A. et al. (2006) Brain 129, 1570.).
      • HeLa transfected cells (1:200) (Kleopa, K.A. et al. (2006) Brain 129, 1570.).
    References
      • KV1.2 is a mammalian voltage-dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.2 was first cloned from rat brain.1 Eight Shaker-related genes exist in mammals constituting the KV1 subfamily of the large KV channel family of genes.2

        A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function.

        The structure of KV1.2 channel is similar to all KV channels and includes six membrane spanning helices creating a voltage sensor domain and a pore domain.2

        The channel is expressed in neurons and cardiac and smooth muscle tissue as well as in retina and pancreas.2 The crystal structure of KV1.2 was recently solved shading light on the structure of a mammalian voltage dependent channel.3 The functional channel is considered low voltage activated and shows very little inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle.

        KV1.2 channels are sensitive to high doses of TEA (560 mM) and low doses of 4-AP (0.59 mM), the “classical” non-selective potassium channel blockers.

        Several venomous toxins from snakes, scorpions and honey bee are potent blockers (affecting the channels in the nanomolar range) of KV1.2 channels. Among these, the most potent and selective are α-Dendrotoxin (1-12 nM), Dendrotoxin-I (0.13 nM), Maurotoxin (0.1-0.8 nM), Hongotoxin-1 (0.17 nM), Margatoxin (0.16-0.65 nM), Tityustoxin Kα (0.21 nM) and MCD peptide (10-400 nM).4

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 08/11/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KV1.2. Anti-KV1.2 (KCNA2) Antibody (#APC-010) can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KV1.2 from human, rat, and mouse samples.

    For research purposes only, not for human use
    Citations
      • Human lung carcinoma cell line A549 isolated nuclei.
        Jang, S.H. et al. (2015) J. Biol. Chem. 290, 12547.
      • Rat lung lysate.
        Lv, Y. et al. (2013) Am. J. Physiol. 305, L856.
      • Mouse spinal cord lysate (1:1000).
        Zoupi, L. et al. (2013) Glia 61, 1236.
      • Rat lumbar spinal cord sections.
        Wolff, M. et al. (2016) Neurosci. Res. 109, 16.
      • Rat DRGs.
        Takahashi, R. et al. (2013) J. Urol. 190, 2296.
      • Mouse spinal cord and cortex sections (1:200).
        Zoupi, L. et al. (2013) Glia 61, 1236.
      • Human artery tissues (1:50).
        Gojkovic-Bukarica, L. et al. (2011) Eur. J. Pharmacol. 654, 266.
      • Mouse cerebellum (1:300).
        Kleopa, K.A. et al. (2006) Brain 129, 1570.
      • Rat hippocampus cell culture.
        Sobieski, C. et al. (2015) J. Neurosci. 35, 11105.
      • HeLa transfected cells (1:200).
        Kleopa, K.A. et al. (2006) Brain 129, 1570.
    Related Products
        • Tityustoxin-Kα-ATTO-594 (#STT-360-AR).
          A fluorescent labeled specific KV1.2 blocker. It can be used in immuno-colocalization studies to label KV1.2 channels.