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This antibody is shipped with its antigen FREE of charge!
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2. Anti-KV1.2 (KCNA2) Antibody, preincubated with the control peptide antigen.
2. Anti-KV1.2 (KCNA2) Antibody, preincubated with the control antigen.
KV1.1 is a mammalian voltage-dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.1 was the first mammalian KV channel to be cloned from mouse brain.1 Eight Shaker-related genes exist in mammals constituting the KV1, subfamily of the large KV channel family of genes.2
A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function.
The structure of KV1.1 channel is similar to all KV channels and includes six membrane spanning helices creating a voltage sensor domain and a pore domain.2
The channel is expressed in neurons and cardiac and skeletal muscle tissue as well as in retina and pancreas.2 The functional channel is considered low voltage activated and shows very little inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle. Mutations in the coding of KV1.1 gene were discovered in Episodic Ataxia patients.3
KV1.1 channels are sensitive to low doses of TEA (0.3 mM) and 4-AP (0.29 mM), the “classical” non-selective potassium channel blockers.
Several venomous toxins from snakes, scorpions and sea anemones are potent blockers (affecting the channels in the nanomolar range) of KV1.1 channels. Among these the most potent and selective are α-Dendrotoxin (#D-350, 0.4-4 nM) and δ-Dendrotoxin (#D-380, 0.03-1.8 nM), Dendrotoxin-K (#D-400, 0.03 nM), Agitoxin-2 (#STA-420, 0.044 nM) and Hongotoxin-1 (#RTH-400, 0.031 nM).4
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KV1.2. Anti-KV1.2 (KCNA2) Antibody (#APC-010) can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KV1.2 from human, rat, and mouse samples.
- Rat lumbar spinal cord sections.
Wolff, M. et al. (2016) Neurosci. Res. 109, 16.
- Rat DRGs.
Takahashi, R. et al. (2013) J. Urol. 190, 2296.
- Mouse spinal cord and cortex sections (1:200).
Zoupi, L. et al. (2013) Glia 61, 1236.
- Human artery tissues (1:50).
Gojkovic-Bukarica, L. et al. (2011) Eur. J. Pharmacol. 654, 266.
- Mouse cerebellum (1:300).
Kleopa, K.A. et al. (2006) Brain 129, 1570.
- Hao, J. et al. (2013) Neuron 77, 899.
- Horn, K.E. et al. (2013) Cell Rep. 3, 173.
- Bin, J.M. et al. (2012) PLoS ONE 7, e41237.
- Cazzin, C. et al. (2011) Genes Brain Behav. 10, 817.
- Gojkovic-Bukarica, L. et al. (2011) Eur. J. Pharmacol. 654, 266.
- Irani, S.R. et al. (2010) Brain 133, 2734.
- Savvaki, M. et al. (2010) J. Neurosci. 30, 13943.
- Sun, W. et al. (2010) J. Neurophysiol. 103, 469.
- Utsunomiya, I. et al. (2010) J. Neurochem. 112, 913.
- Hayashi, Y. et al. (2009) Am J. Physiol. 296, R1661.
- Hsiao, C.F. et al. (2009) J. Neurophysiol. 101, 1407.
- Cox, R.H. et al. (2008) Am. J. Hypertens. 21, 213.
- Gautier, M. et al. (2007) Am. J. Physiol. 292, 475.
- Neshatian, L. et al. (2007) Am. J. Physiol. 292, G1233.
- Rivera, J. et al. (2007) Eur. J. Neurosci. 25, 136.
- Susuki, K. et al. (2007) Glia 55, 746.
- Kuba, H. et al. (2006) Nature 444, 1069.
- Fordyce, C.B. et al. (2005) J. Neurosci. 25, 7139.
- Kuba, H. et al. (2005) J. Neurosci. 25, 1924.
- Wang, J. et al. (2005) Am. J. Physiol. 288, L1049.
- Karimi-Abdolrezaee, S. et al. (2004) Eur. J. Neurosci. 19, 577.
- Nakayama, H. et al. (2004) J. Neurosci. 24, 3199.
- Dodson, P.D. et al. (2003) J. Physiol. 550, 27.
- Popratiloff, A. et al. (2003) J. Comp. Neurol. 461, 466.
- Rios, J.C. et al. (2003) J. Neurosci. 23, 7001.
- Adamson, C.L. et al (2002) J. Neurosci. 22, 1385.
- Arroyo, E.J. et al. (2002) J. Neurosci. 22, 1726.
- Felix, R. et al. (2002) Zygote 10, 183.
- Chung, Y.H. et al. (2000) Brain Res. 875, 164.
- Conforti, L. et al. (2000) J. Physiol. 524, 783.
- Nashmi, R. et al. (2000) Eur. J. Neurosci. 12, 491.
- Arroyo, E.J. et al. (1999) J. Neurocytol. 28, 333.
- Sobko, A. et al. (1998) J. Neurosci. 18, 10398.
- Yuan, X.J. et al. (1998) Am. J. Physiol. 274, L621.
- Attali, B. et al. (1997) J. Neurosci. 17, 8234.
- Wang, J. et al. (1997) J. Clin. Invest. 100, 2347.
Products for control experiments
- Anti-KV1.2 (KCNA2) (extracellular) Antibody (#APC-162).
This antibody recognizes a different epitope and thus can also be used as a control for detecting KV1.2.
- KV1.2 (KCNA2) Channel Overexpressed Membrane Fractions (#LX-101).
The KV1.2 overexpressed membrane fractions enables end users to check the expression of endogenous KV1.2 as opposed to a tagged version and serves as a positive control for testing the expression of the channel and a quick way to test the antibody.
- KV1.2 (KCNA2) Channel Antibody and Membrane Fractions Kit (#LK-101).
This kit includes the KV1.2 overexpressed membrane fractions, and Anti-KV1.2 (KCNA2) Antibody (#APC-010).
Antibodies for immuno-colocalization experiments
- Tityustoxin-Kα-ATTO-594 (#STT-360-AR).
A fluorescent labeled specific KV1.2 blocker. It can be used in immuno-colocalization studies to label KV1.2 channels.
- Antibodies for live cell imaging experiments
- Anti-KV1.3 (KCNA3) Antibody (#APC-002)
- Anti-KV1.5 (KCNA5) (extracellular) Antibody (#APC-150)
- α-Dendrotoxin (#D-350)
- Hemitoxin (#STH-500)
- Spinoxin (#STS-500)
- Tityustoxin-Kα (#STT-360)
- Urotoxin (#STU-200)
- Shaker (KV1) Channel Antibody Explorer Kit (#AK-219)
- Shaker (KV1) Channel Blocker Explorer Kit (#EK-107)