- Peptide (C)KLEDHEYKPLDPKDIR, corresponding to amino acid residues 669-684 of mouse CHERP (Accession Q8CGZ0). Cytoplasm.
- Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and mouse C2C12 muscle myoblast cell line (lanes 3 and 6) lysates:1-3. Anti-CHERP Antibody (#ACC-330), (1:1500).
4-6. Anti-CHERP Antibody, preincubated with CHERP Blocking Peptide (#BLP-CC330).
- Expression of CHERP in mouse brainImmunohistochemical staining of mouse brain sections using Anti-CHERP Antibody (#ACC-330). A. In mouse hippocampal CA3 region, CHERP immunoreactivity (green) appears in neurons (arrows) of CA3 pyramidal layer (P). B. In parietal cortex, CHERP staining (green) is detected in neurons (arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).
CHERP (calcium homeostasis endoplasmic reticulum protein) is a ubiquitously expressed integral sarco/endoplasmic reticulum (SR/ER) membrane protein. CHERP protein consist of 916 amino acid in humans and its structure containing a Pro-rich region and a segment of Arg-Ser dipeptide repeats (an RS domain) near the C terminus, and an RNA recognition motifs (RRMs)1-3.
CHERP acts as a regulator of both major families of endoplasmic reticulum Ca2+ channels, inositol 1,4,5-trisphosphate receptors (IP3Rs) and ryanodine receptors (RyRs). CHERP is responsible for the regulation of intracellular Ca2+ mobilization in human cells and is involved in neuroblastoma cell proliferation, apoptosis and tumorigenicity1,2.
CHERP co-localizes with IP3 receptors throughout the cytoplasmic and perinuclear regions in human erythroleukemia cells and in Jurkat cells. CHERP is also detected in the nucleoplasm with prominent accumulation at nuclear speckles1,2. CHERP co-localizes with endogenous ryanodine receptor type 1 (RyR1) in the sarcoplasmic reticulum of rat soleus muscle. Studies of CHERP knockdown reveal decreased intracellular Ca2+ mobilization, cell growth and proliferation2,3.
Species reactivity key:
Anti-CHERP Antibody (#ACC-330) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CHERP from human, rat, and mouse samples.
- Anti-CHERP-ATTO Fluor-594 Antibody (#ACC-330-AR).
A fluorescent labeled primary antibody. It can be used in multiplex staining studies in conjunction with any of our antibodies raised in rabbit.
- Anti-CALHM1 Antibody (#ACC-101)
- Anti-CALHM1-ATTO Fluor-594 Antibody (#ACC-101-AR)
- Anti-IP3 Receptor-1 (ITPR1) Antibody (#ACC-019)
- Anti-IP3 Receptor-2 (ITPR2) Antibody (#ACC-116)
- Anti-IP3 Receptor-2 (ITPR2)-ATTO Fluor-594 Antibody (#ACC-116-AR)
- Anti-Ryanodine Receptor 1 Antibody (#ARR-001)
- Anti-Ryanodine Receptor 2 Antibody (#ARR-002)
- Anti-Ryanodine Receptor 3 Antibody (#ARR-003)