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Anti-Ryanodine Receptor 2 Antibody

RyR2, Cardiac muscle ryanodine receptor
Cat #: ARR-002
Alternative Name RyR2, Cardiac muscle ryanodine receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CAGESMSPGQGRNN, corresponding to amino acid residues 1489-1502 of human RyR2 (Accession Q92736). Intracellular, N-terminus.
Accession (Uniprot) Number Q92736
Gene ID 6262
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse - identical.
RRID AB_2040184.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat heart membranes:
    Western blot analysis of rat heart membranes:
    1. Anti-Ryanodine Receptor 2 Antibody (#ARR-002), (1:200).
    2. Anti-Ryanodine Receptor 2 Antibody, preincubated with Ryanodine Receptor 2 Blocking Peptide (#BLP-RR002).
Immunohistochemistry
  • Expression of RyR2 in rat cardiac muscle
    Expression of RyR2 in rat cardiac muscle
    Immunohistochemical staining of paraffin-embedded sections of rat myocardium using Anti-Ryanodine Receptor 2 Antibody (#ARR-002), (1:50). Staining is specific for cardiomyocytes while smooth muscles cells in the artery walls are negative (red arrows). Hematoxilin is used as the counterstain.
  • Expression of RyR2 in mouse cerebellum
    Expression of RyR2 in mouse cerebellum
    Immunohistochemical staining of mouse cerebellum frozen sections with Anti-Ryanodine Receptor 2 Antibody (#ARR-002), (1:100), (green fluorescence). A. The highest expression of RyR2 is in the molecular layer (asterisk) but there is also some expression in the soma of Purkinje cells (arrows). B. In the same section, there is staining for parvalbumin (red), a marker for Purkinje cells. C. Merged image of panels A and B demonstrates that RyR2 is localized both in the area surrounding the dendritic tree and in the soma of Purkinje cells. DAPI is used as the counterstain (blue).
Immunocytochemistry
References
  1. Chakrabarti, R. and Chakrabarti, R. (2006) J. Cell. Biochem. 99, 1503.
  2. Eisner, D.A. et al. (2005) Exp. Physiol. 90, 3.
  3. Bers, D.M. (2004) J. Mol. Cell. Cardiol. 37, 417.
  4. Fill, M. and Copello, J.A. (2002) Physiol. Rev. 82, 893.
Scientific background

It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2  The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.

The ER and SR contain two Ca2+ release channel families, the inositol trisphosphate receptors (IP3Rs) and the ryanodine receptors (RyRs).3

The ryanodine receptor family consists of three different isoforms: the skeletal muscle isoform, ryanodine receptor type 1 (RyR1); the cardiac muscle isoform, ryanodine receptor type 2 (RyR2) and the brain isoform, ryanodine receptor type 3 (RyR3).3

The ryanodine receptors are homotetrameric proteins, which upon assembly create a Ca2+ conducting channel. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of ryanodine (a poisonous alkaloid found in the South American plant Ryania speciosa) to the ryanodine receptor causes two major changes in the channel: a reduction in single-channel conductance and a marked increase in the open state probability, leading to an overall increase/decrease in the Ca2+ release capability of the channel.

RyR2 serves as an intracellular Ca2+ channel in the SR membrane. It is predominantly expressed in cardiac muscle where it plays a central role in cardiac excitation-contraction coupling. RyR2 is also expressed in the brain.1-4

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title:

Anti-Ryanodine Receptor 2 Antibody
Expression of RyR2 in mouse hippocampal neurons.Immunocytochemical staining of mouse primary hippocampal neurons using Anti-Ryanodine Receptor 2 Antibody (#ARR-002).Adapted from Gan, K.J. and Silverman, M.A. (2015) Mol. Biol. Cell 26, 1058. with permission of the American Society for Cell Biology.

Last update: 23/08/2020

Anti-Ryanodine Receptor 2 Antibody (#ARR-002) is a highly specific antibody directed against an epitope of human RyR2. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize RyR2 from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Mouse heart lysate.
    Yang, L. et al. (2016) Proc. Natl. Acad. Sci. U.S.A. 113, E7976.
  2. Rat intrapulmonary artery lysate (1:400).
    Dahan, D. et al. (2012) Am. J. Physiol. 303, L824.
Immunohistochemistry citations
  1. Rat heart sections (1:50).
    Zhu-Mauldin, X. et al. (2012) J. Biol. Chem. 287, 39094.
  2. Rat tibialis anterior muscle sections (1:100).
    Kraner, S.D. et al. (2011) Am. J. Physiol. 300, R1384.
Immunocytochemistry citations
  1. Mouse isolated vascular smooth muscle cells.
    Thakore, P. et al. (2020) Sci. Signal. 13, eaba1015.
  2. Rat adipose-derived stromal cells and rat bone marrow stromal cells.
    Forostyak, O. et al. (2016) Stem Cell Res. 16, 622.
  3. Mouse primary hippocampal neurons (1:300).
    Gan, K.J. and Silverman, M.A. (2015) Mol. Biol. Cell 26, 1058.
More product citations
  1. Yoshida, S. et al. (2010) Neurosci. Res. 68, 322.
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