Anti-Ryanodine Receptor 2

Cardiac Ryanodine Receptor, RyR2
    Cat #: ARR-002
    Sizes: 25 µl, 50 µl, 0.2 ml
  • Lyophilized Powder
  • Antigen Incl.
  • QC Tested
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide CAGESMSPGQGRNN, corresponding to amino acid residues 1489-1502 of human Ryanodine Receptor 2 (Accession Q92736). Intracellular, N-terminus.
    Gene ID Q92736
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, wb
    May also work in: ifc*, ip*
    Western blot
    Western blot analysis of rat heart membranes:
    1. Anti-Ryanodine Receptor 2 antibody (#ARR-002), (1:200).
    2. Anti-Ryanodine Receptor 2 antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of Ryanodine Receptor 2  in rat cardiac muscle
    Immunohistochemical staining of paraffin-embedded sections of rat myocardium using Anti-Ryanodine Receptor 2 antibody (#ARR-002), (1:50). Staining is specific for cardiomyocytes while smooth muscles cells in the artery walls are negative (red arrows). Hematoxilin is used as the counterstain.
    Expression of Ryanodine Receptor 2 in mouse cerebellum
    Immunohistochemical staining of mouse cerebellum frozen sections with Anti-Ryanodine Receptor 2 antibody (#ARR-002), (1:100), (green fluorescence). A. The highest expression of Ryanodine Receptor 2 is in the molecular layer (Asterisk) but there is also some expression in the soma of Purkinje cells (arrows). B. In the same section, there is staining for parvalbumin (red), a marker for Purkinje cells. C. Merged image of panels A and B demonstrates that Ryanodine Receptor 2 is localized both in the area surrounding the dendritic tree and in the soma of Purkinje cells. DAPI is used as the counterstain (blue).
    Immunocytochemistry
    Mouse primary hippocampal neurons (1:300) (see Gan, K.J. and Silverman, M.A. (2015) Mol. Biol. Cell 26, 1058.)
    References
    1. Chakrabarti, R. And Chakrabarti, R. (2006) J.Cell. Biochem99, 1503.
    2. Eisner, D.A. et al. (2005) Exp.Physiol. 90, 3.
    3. Bers, D.M. (2004) J.Mol. Cell. Cardiol. 37, 417.
    4. Fill, M. and Copello, J.A. (2002) Physiol. Rev. 82, 893.
    Scientific background

    It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2  The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.

    The ER and SR contain two Ca2+ release channels families, the Inositol trisphosphate receptors (IP3Rs) and the Ryanodine receptors (RyRs).3

    The Ryanodine receptor family consists of three different isoforms: The skeletal muscle isoform, Ryanodine Receptor type 1 (RyR1); the cardiac muscle isoform, Ryanodine Receptor type 2 (RyR2) and the brain isoform, Ryanodine Receptor type 3 (RyR3).3 The Ryanodine receptors are homotetrameric proteins. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of Ryanodine to the Ryanodine Receptor causes to two major changes in the channel: a reduction in single-channel conductance and a marked increase in open state probability.

    RyR2 serves as an intracellular Ca2+ channel in the SR membrane. It is predominantly expressed in cardiac muscle where it plays a central role in cardiac excitation-contraction coupling. RyR2 is also expressed in the brain. 1-4

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:


    Expression of RyR2 in mouse hippocampal neurons.Immunocytochemical staining of mouse primary hippocampal neurons using Anti-Ryanodine Receptor 2 antibody (#ARR-002).Adapted from Gan, K.J. and Silverman, M.A. (2015) Mol. Biol. Cell 26, 1058. with permission of the American Society for Cell Biology.

    Last update: 25/12/2017

    Anti-Ryanodine Receptor 2 (#ARR-002) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize RyR2 from human, rat, and mouse samples.

    For research purposes only, not for human use

    For a list of product citations in the literature, see “product citations” below. If you know of additional relevant citations for this product, please let us know.

    Citations
    Western blot citations
    1. Mouse heart lysate.
      Yang, L. et al. (2016) Proc. Natl. Acad. Sci. U.S.A. In press.
    2. Rat intrapulmonary artery lysate (1:400).
      Dahan, Det al. (2012) Am. J. Physiol. 303, L824.
    Immunohistochemistry citations
    1. Rat heart sections (1:50).
      Zhu-Mauldin, X. et al. (2012) J. Biol. Chem. 287, 39094.
    2. Rat tibialis anterior muscle sections (1:100).
      Kraner, S.Det al. (2011) Am. J. Physiol. 300, R1384.
    Immunocytochemistry citations
    1. Rat adipose-derived stromal cells and rat bone marrow stromal cells.
      Forostyak, O. et al. (2016) Stem Cell Res. 16, 622.
    2. Mouse primary hippocampal neurons (1:300).
      Gan, K.J. and Silverman, M.A. (2015) Mol. Biol. Cell 26, 1058.
    More product citations
    1. Yoshida, S. et al. (2010) Neurosci. Res. 68, 322.
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