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2. Anti-Ryanodine Receptor 2 antibody, preincubated with the control peptide antigen.
It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.
The ER and SR contain two Ca2+ release channels families, the Inositol trisphosphate receptors (IP3Rs) and the Ryanodine receptors (RyRs).3
The Ryanodine receptor family consists of three different isoforms: The skeletal muscle isoform, Ryanodine Receptor type 1 (RyR1); the cardiac muscle isoform, Ryanodine Receptor type 2 (RyR2) and the brain isoform, Ryanodine Receptor type 3 (RyR3).3 The Ryanodine receptors are homotetrameric proteins. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of Ryanodine to the Ryanodine Receptor causes to two major changes in the channel: a reduction in single-channel conductance and a marked increase in open state probability.
RyR2 serves as an intracellular Ca2+ channel in the SR membrane. It is predominantly expressed in cardiac muscle where it plays a central role in cardiac excitation-contraction coupling. RyR2 is also expressed in the brain. 1-4
Species reactivity key:
Expression of RyR2 in mouse hippocampal neurons.Immunocytochemical staining of mouse primary hippocampal neurons using Anti-Ryanodine Receptor 2 antibody (#ARR-002).Adapted from Gan, K.J. and Silverman, M.A. (2015) Mol. Biol. Cell 26, 1058. with permission of the American Society for Cell Biology.
Anti-Ryanodine Receptor 2 (#ARR-002) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize RyR2 from human, rat, and mouse samples.
For a list of product citations in the literature, see product citations tab below. If you know of additional relevant citations for this product, please let us know.
For a list of product citations in the literature, see “product citations” below. If you know of additional relevant citations for this product, please let us know.
- Yoshida, S. et al. (2010) Neurosci. Res. 68, 322.