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Anti-Dopamine Transporter (DAT) (extracellular)-FITC Antibody

Sodium-dependent dopamine transporter, DA transporter, Solute carrier family 6 member 3, SLC6A3

Cat #: AMT-003-F
Alternative Name Sodium-dependent dopamine transporter, DA transporter, Solute carrier family 6 member 3, SLC6A3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)DAHASNSSDGLGLND, corresponding to amino acids residues 191-205 of rat DAT (Accession P23977). 2nd extracellular loop.
Accession (Uniprot) Number P23977
Gene ID 24898
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – 14/15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Not recommended for use with human samples.
Label Fluorescein isothiocyanate (FITC).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AMT-003), and direct flow cytometry (labeled antibody).
Applications: fc
May also work in: ic*, lci*
Direct flow cytometry
  • Cell surface detection of DAT in mouse J774 macrophages:
    Cell surface detection of DAT in mouse J774 macrophages:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Dopamine Transporter (DAT) (extracellular)-FITC Antibody (#AMT-003-F), (5 µg).
  • Cell surface detection of DAT in mouse TK-1 T-cell lymphoma cells:
    Cell surface detection of DAT in mouse TK-1 T-cell lymphoma cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Dopamine Transporter (DAT) (extracellular)-FITC Antibody (#AMT-003-F), (5 µg).
References
  1. Robertson, S.D. et al. (2009) Mol. Neurobiol. 39, 73.
  2. Gainetdinov, R.R. (2008) Nauyn-Schiedeberg’s Arch. Pharmacol. 377, 301.
  3. Torres, G.E. and Amara, S.G. (2007) Curr. Opin. Neurobiol. 17, 304.
  4. Ciliax, B.J. et al. (1995) J. Neurosci. 15, 1714.
  5. Uhl, G.R. (2003) Mov. Disord. 18, S71.
Scientific background

The Na+/Cl- transporter family SLC6, includes DAT, a monoamine transporter, important for regulating extracellular levels of dopamine. It does so by taking up dopamine from the synaptic cleft via the co-transport of Na+ and Cl- down their electrochemical gradients1. The removal of dopamine by DAT remains the most important means to control the extracellular lifetime of the neurotransmitter and notably, the ending of dopaminergic neurotransmission2. Transporters for serotonin, norepinephrine, GABA and glycine also belong to this family3.

These receptors have 12 transmembrane spanning domains and intracellular N- and C-termini. DAT is also subject to post translational modifications such as phosphorylation, important for its regulation1. It also possesses a large extracellular domain which undergoes N-glycosylation, important for the proper targeting of the transporter to the plasma membrane1. DAT is expressed in dopaminergic cell bodies and terminals and can therefore serve as a marker for these neurons4. DAT is also expressed in the retina, gastrointestinal tract, lung, kidney, pancreas and lymphocytes2.

DAT plays an important role in movement as well as reward, learning and memory5. Its malfunction, which leads to dopaminergic dysregulation, has been associated with ADHD, schizophrenia, as well as Parkinson’s disease2. DAT is the main target for cocaine, amphetamine and methamphetamine psychostimulants, which mainly increase locomotor activities2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/03/2021

Anti-Dopamine Transporter (DAT) (extracellular) Antibody (#AMT-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize DAT from mouse and rat samples. The antibody is unlikely to recognize DAT from human samples.

Anti-Dopamine Transporter (DAT) (extracellular)-FITC Antibody (#AMT-003-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

For research purposes only, not for human use
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