Free shipping starts now, no minimum, no coupons required!

Anti-EphA4 (extracellular) Antibody

Ephrin Type-A Receptor 4, EPH-Like Kinase 8, EPHA4

Cat #: AER-044
Alternative Name Ephrin Type-A Receptor 4, EPH-Like Kinase 8, EPHA4
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)ERSYRIVRTAARNTD, corresponding to amino acid residues 485 - 499 of human EPHA4 (Accession P54764). Extracellular, N-terminus.
Accession (Uniprot) Number P54764
Gene ID 2043
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat – identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Applications: ifc, lci, wb
May also work in: ic*, ih*
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1-2. Anti-EphA4 (extracellular) Antibody (#AER-044), (1:200).
    3-4. Anti-EphA4 (extracellular) Antibody, preincubated with EphA4 (extracellular) Blocking Peptide (BLP-ER044).
  • Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 5), human U-87 MG glioblastoma cell line lysate (lanes 2 and 6), human Jurkat T-cell leukemia cell line lysate (lanes 3 and 7) and human THP-1 monocytic leukemia cell line lysate (lanes 4 and 8):
    Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 5), human U-87 MG glioblastoma cell line lysate (lanes 2 and 6), human Jurkat T-cell leukemia cell line lysate (lanes 3 and 7) and human THP-1 monocytic leukemia cell line lysate (lanes 4 and 8):
    1-4. Anti-EphA4 (extracellular) Antibody (#AER-044), (1:200).
    5-8. Anti-EphA4 (extracellular) Antibody, preincubated with EphA4 (extracellular) Blocking Peptide (BLP-ER044).
Indirect flow cytometry
  • Cell surface detection of EphA4 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    Cell surface detection of EphA4 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-EphA4 (extracellular) Antibody (#AER-044), (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Rüdiger Klein (2012), Development, 139, 4105.
  2. Chen et al (2015), PLoS One., 10, e0126942.
  3. Clifford et al (2011), Neural development., 6, 21.
  4. Greferath et al (2002), Mech Dev. , 119, S231.
Scientific background

EphA4 is a member of the Eph receptor tyrosine kinase family.

Eph receptors are subdivided into two subclasses, termed EphA and EphB, based on sequence similarity and their preference for binding a particular subclass of ephrins. The domain organization of Eph receptors contains a globular ligand binding domain (LBD), a cysteine-rich region, and two fibronectin type III domains (FN1 and FN2). It also contains a transmembrane (TM) helix, and an intracellular part consisting of a juxtamembrane (JM) region and a tyrosine kinase domain. Eph kinase activity is auto-inhibited through interaction with its own JM region, this auto inhibition is released by phosphorylation1.

Interactions of Eph receptors with their ligands, ephrins, at cell-cell interfaces promote a variety of cellular responses, including repulsion, attraction and migration2.

EphA4 is activated by all members of the ephrin-A ligand family and most members of the ephrin-B family1. Upon ligand binding it activates pathways that regulate the formation of the axon tract and cortical network as well as radial migration of cortical neurons in the cortex3.

EphA4 is expressed in the neural ectoderm at embryonic day 8 and then in various cell types throughout the cortex during subsequent stages of neurodevelopment4, its major effect on cell-cell interfaces makes this receptor highly important for the study of cellular regeneration and various types of disease.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 27/06/2021

Anti-EphA4 (extracellular) Antibody (#AER-044) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize EPHA4 from mouse, rat and human samples.

For research purposes only, not for human use
Shipping and Ordering information