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Anti-GABA(A) π Receptor (extracellular) Antibody

GABA(A) receptor subunit pi, Gabrp, Gamma-aminobutyric acid receptor subunit pi
Cat #: AGA-019
Alternative Name GABA(A) receptor subunit pi, Gabrp, Gamma-aminobutyric acid receptor subunit pi
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)DGNDVEFSWLRGND, corresponding to amino acid residues 184-197 of rat GABA(A) π Receptor (Accession O09028). Extracellular, N-terminus.
Accession (Uniprot) Number O09028
Gene ID 81658
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; human – 13/14 amino acid residues identical.
RRID AB_2876802
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
    Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
    1, 2. Anti-GABA(A) π Receptor/GABRP (extracellular) Antibody (#AGA-019), (1:200).
    3, 4. Anti-GABA(A) π Receptor/GABRP (extracellular) Antibody, preincubated with GABA(A) π Receptor (extracellular) Blocking Peptide (#BLP-GA019).
References
  1. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62, 1.
  2. Chua, H.C. and Chebib, M. (2017) Adv. Pharmacol. 79, 1.
  3. Chen, Z.W. et al. (2019) PloS Biol. 17, e3000157.
  4. Olsen, R.W. (2018) Neuropharmacology 136, 10.
Scientific background

The GABA(A) receptor (GABAAR) is a pentameric transmembrane protein, member of the Cys-loop family of neurotransmitter-gated ion channels. Its ligand is γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. Binding of GABA induces the opening of an anion-selective ion channel that is an integral part of the receptor protein. The opening of this channel enables the entrance or exit of Cl- ions according to the resting potential of the cell surroundings. This causes an inhibitory effect on neurotransmission by decreasing the chance of a successful action potential transmission1. GABAARs occur in all organisms that have a nervous system, and they are mostly located in neuronal tissues. Due to their wide distribution within the nervous system and their basic function, they play a role in virtually all brain functions.

These pentameric receptors occur with diverse subunit composition, at least 19 GABAAR subunits are identified in the human genome and the heteromeric nature of GABAAR allows for an enormous range of theoretically possible subunit combinations. Yet, experimental evidence suggests that only a few dozen combinations exist in vivo2.

Members of the cys-loop family of ion channels share a similar structure that consists of an extracellular N- and C-terminal domains, four helical membrane-spanning segments (M1, M2, M3, and M4) and a 15-residue disulfide-linked loop in the extracellular domain3.

The pivotal role of GABAARs in all brain functions makes them a desirable drug target and an impressive range of clinically used therapeutics are known to bind to distinct sites found on GABAARs to modulate receptor function4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 21/10/2020

Anti-GABA(A) π Receptor/GABRP (extracellular) Antibody (#AGA-019) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize GABRP from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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