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Anti-GABA(A) α5 Receptor Antibody

γ-Aminobutyric acid receptor type A subunit α5, GABRA5

Cat #: AGA-025
Alternative Name γ-Aminobutyric acid receptor type A subunit α5, GABRA5
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)KKERELILNKSTN, corresponding to amino acid residues 364-376 of rat GABRA5 (Accession P19969). 2nd intracellular loop.
Accession (Uniprot) Number P19969
Gene ID 29707
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; human – 11/13 amino acid residues identical.
RRID AB_2756595.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat hippocampus (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
    Western blot analysis of rat hippocampus (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
    1,2. Anti-GABA(A) α5 Receptor Antibody (#AGA-025), (1:400).
    3,4. Anti-GABA(A) α5 Receptor Antibody, preincubated with GABA(A) α5 Receptor Blocking Peptide (#BLP-GA025).
Immunohistochemistry
  • Expression of GABRA5 in mouse brain
    Expression of GABRA5 in mouse brain
    Immunohistochemical staining of mouse hippocampus sections using Anti-GABA(A) α5 Receptor Antibody (#AGA-025), (1;600). A. GABRA5 staining (green). B. Merged image of GABRA5 (green) with nuclei staining using DAPI as the counterstain (blue). GABRA5 is detected in the pyramidal layer (Pyr) (horizontal arrows) and in some interneurons (vertical arrow).
References
  1. Sigel, E. and Steinmann, M.E. (2012) J. Biol. Chem. 287, 40224.
  2. Glatt, K. et al. (1997) Genomics 41, 63.
  3. Zurek, A.A. et al. (2016) Ann. Clin. Trans. Neurol. 3, 392.
Scientific background

GABA(A) receptors are the main inhibitory neurotransmitter receptors in the mammalian brain. They respond to a wide variety of drugs including benzodiazepines. The entire GABA receptor family contains 19 known genes.

GABA(A) receptors are mostly pentameric proteins composed of different subunits and each subunit has several subtypes. Half of each subunit consists of a hydrophilic extracellular N-terminal domain containing a Cys loop, followed by four transmembrane sequences (M1-M4). M2 lines the ion channel and between M3 and M4 there is a large intracellular loop involved in receptor modulation by phosphorylation. Five subunits form a complex and surround a central ion channel with all five M2 segments contributing to the channel1.

The gene encoding the GABA(A) receptor subunit α-5 (also known as GABRA5) is mapped to chromosome 15q11-q13 (together with GABRB3 and GABRG3) and is contained within approximately 800 kb of the distal part of the imprinted Prader-Willi and Angleman syndrome region. The receptor is mostly expressed in the hippocampus and prefrontal cortex2. GABAR5 is anchored to neuronal extra synaptic regions by the cytosolic protein radixin.

GABRA5 is implicated in the development of various social disorders. PET studies have shown reduced binding of a GABRA5 selective ligand in the amygdala and nucleus acumbens of people with Autism spectrum disorders (ASD). In addition, post-mortem analysis of brain tissue obtained from individuals with ASD shows reduced amounts of GABRA5 encoding DNA. Deletion of the GABRA5 gene in mice causes autism-like behavior3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat GABA(A) α5 receptor. Anti-GABA(A) α5 Receptor Antibody (#AGA-025) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABRA5 from human, rat, and mouse samples.

For research purposes only, not for human use
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