Anti-GLP1R (extracellular) Antibody

Glucagon-like peptide 1 receptor, GLP-1 receptor
    Cat #: AGR-021
    Alternative Name Glucagon-like peptide 1 receptor, GLP-1 receptor
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)KYLYEDEG(S)WTRNS, corresponding to amino acid residues 288-301 of rat GLP1R (Accession P32301). 2nd extracellular loop.
        Anti-GLP1R (extracellular) Antibody
    Accession (Uniprot) Number P32301
    Gene ID 25051
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse, human - 13/14 amino acid residues identical.
    RRID AB_10917158.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, lci, wb
    May also work in: ifc*, ip*
      • Anti-GLP1R (extracellular) Antibody
        Western blot analysis of rat pancreas lysate (lanes 1 and 5), mouse preadipocyte 3T3-L1 lysate (lanes 2 and 6), rat pancreatic islet cell line RIN-5F lysate (lanes 3 and 7) and human pancreatic carcinoma PANC-1 lysate (lanes 4 and 8):
        1-4. Anti-GLP1R (extracellular) Antibody (#AGR-021), (1:200).
        5-8. Anti-GLP1R (extracellular) Antibody, preincubated with the negative control antigen.
      • Anti-GLP1R (extracellular) Antibody
        Expression of Glucagon-like peptide 1 receptor in rat pancreas
        Immunohistochemical staining of rat pancreas paraffin embedded sections using Anti-GLP1R (extracellular) Antibody (#AGR-021), (1:100). Staining (brown color) is present in endocrine cells of the Isles of Langerhans. Hematoxilin is used as the counterstain.
        Anti-GLP1R (extracellular) Antibody
        Expression of Glucagon-like peptide 1 receptor in rat hypothalamus
        Immunohistochemical staining of rat hypothalamus with Anti-GLP1R (extracellular) Antibody (#AGR-021), (1:200). Glucagon-like peptide 1 receptor (green) appears in the lateral hypothalamic region axonal profiles (vertical arrow) and nerve cell profiles (horizontal arrows). DAPI is used as the counterstain (blue).
      • Anti-GLP1R (extracellular) Antibody
        Expression of Glucagon-like peptide 1 receptor in rat pancreatic islet cells
        Cell surface detection of Glucagon-like peptide 1 receptor in live intact rat RIN-5F pancreatic islet cells. A. Cells were stained with Anti-GLP1R (extracellular) Antibody (#AGR-021), (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized with the membrane-permeable DNA dye Hoechst 33342 (blue staining). C. Live view of the cells.
    References
      • Glucagon-like peptide 1 (GLP-1) is a hormone secreted by the intestine following a meal1. This hormone is known to enhance the secretion of insulin, important glucose blood level regulation2,3. In pancreatic β-cells, other than stimulating insulin secretion, GLP-1 action is also important for β-cell mass expansion2.

        GLP-1 exerts its biological functions through Glucagon-like peptide 1 receptor (GLP-1 receptor), a member of the G-protein coupled receptor superfamily. Like all members, this receptor spans the membrane seven times. GLP-1 receptor couples with Gs, thereby stimulating adenylate cyclase, consequently leading to an increase in cAMP2.

        The receptor is expressed in various tissues, namely the pancreas, adipose tissue, muscle, heart, gastrointestinal tract and liver. GLP-1 receptor is also found in the central nervous system where it provides a neuroprotective effect4.

        The glucagon-like peptide 1 system may serve as a therapeutic treatment in diabetes mellitus, in order to maintain β-cell mass expansion2. In addition, the receptor may serve as a therapy target in various neurodegenerative diseases like Alzheimer’s and Parkinson’s disease4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-GLP1R (extracellular) Antibody (#AGR-021) is a highly specific antibody directed against an epitope of the rat glucagon-like peptide 1 receptor. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and live cell imaging applications. It has been designed to recognize GLP1R from human, rat, and mouse samples.

    For research purposes only, not for human use